山东医药2024，Vol.64Issue(11)：17-20,4.DOI:10.3969/j.issn.1002-266X.2024.11.004
肝缺血再灌注对幼鼠血脑屏障通透性及脑组织细胞凋亡的影响
Effects of hepatic ischemia-reperfusion on blood-brain barrier permeability and brain tissue apoptosis in young mice
摘要
Abstract
Objective To observe the effects of hepatic ischemia-reperfusion(HIR)on blood-brain barrier(BBB)permeability and brain tissue apoptosis in young mice.Methods Twenty-four healthy and clean grade C57BL/6 young mice were randomly divided into the sham operation group,dextran glycoside 10 group,and dextran glycoside 40 group,with 8 mice in each group.The HIR models were established by clamping the left hepatic artery and portal vein in mice of both dextran glycoside 10 and 40 groups.After 60 min of reperfusion,dextran glycoside with a molecular weight of 10 and 40 Kd was injected into the inferior vena cava,respectively.Mice in the sham operation group only underwent abdominal opening and closing,free blood vessels,and other procedures.Immunofluorescence staining was used to observe the per-meability of brain tissue BBB(expressed as the pass rate of dextran glycoside),Western blotting was used to detect the in-dicators related to BBB tight junction protein in brain tissues,including Claudin,Occludin and β-Catenin and tight junc-tion protein 1(ZO-1),and TUNEL method was used to detect the apoptosis rate of brain tissues.Results Compared with the sham operation group,the dextran glycoside 10 group and the dextran glycoside 40 group had higher passing rates of dextran glycoside and apoptosis rates in the brain tissues of young mice,and the low-dose group had a higher passing rate of dextran glycoside(all P<0.05).Compared with the sham operation group,the relative expression levels of Clau-din,Occludin,and β-Catenin and ZO-1 proteins decreased(all P<0.05),and there were no statistically significant differ-ences in the above indicators between the dextran glycoside 10 and 40 groups(all P>0.05).Conclusion HIR can lead to increased BBB permeability and increased apoptosis of brain tissues in young mice.关键词
肝脏/缺血再灌注损伤/血脑屏障/幼鼠Key words
liver hepatic ischemia reperfusion injury/blood-brain barrier/young mice分类
医药卫生引用本文复制引用
贾莉莉,喻文立,张涛,吕丹..肝缺血再灌注对幼鼠血脑屏障通透性及脑组织细胞凋亡的影响[J].山东医药,2024,64(11):17-20,4.基金项目
国家自然科学基金面上项目(82072219) （82072219）
天津市卫生健康科技项目(TJWJ2023QN025) （TJWJ2023QN025）
天津市自然科学基金面上项目(21JCYB-JC00150). （21JCYB-JC00150）
