山东医药2024，Vol.64Issue(13)：26-29,35,5.DOI:10.3969/j.issn.1002-266X.2024.13.006
口腔扁平苔藓患者血清miR-155-5p、SOCS1 mRNA水平与病损程度及Th17/Treg失衡的关系
Relationships between miR-155-5p and SOCS1 levels and the degree of lesion and Th17/Treg imbalance in OLP patients
摘要
Abstract
Objective To investigate the relationships between the levels of serum microRNA-155-5p(miR-155-5p)and suppressor of cytokine signaling1(SOCS1)and the degree of lesion and the imbalance of helper T cell 17(Th17)/reg-ulatory T cell(Treg)in oral lichen planus(OLP)patients.Methods Seventy-six OLP patients(observation group)were selected,including 28 non-erosive OLP patients and 48 erosive OLP patients(29 mild to moderate erosive OLP patients,19 severe erosive OLP patients),and meanwhile,50 healthy volunteers(control group)were randomly selected during the same period.Peripheral venous blood of all subjects was collected.Real-time fluorescence quantitative PCR was used to detect serum miR-155-5p and SOCS1 mRNA,and flow cytometry was used to detect the of Th17 ratio and Treg ratio,and to calculate the Th17/Treg.Pearson/Spearman correlation analysis was used to analyze the relationship between serum miR-155-5p level and SOCS1 mRNA level,and their relationships with the ratios of Th17,Treg and Th17/Treg in the peripheral blood.Results Compared with the control group,the serum miR-155-5p level,Th17 ratio and Th17/Treg in peripheral blood increased,while the serum SOCS1 mRNA level and Treg ratio in peripheral blood decreased in the observation group(all P＜0.05).In patients with non-erosive,mile-to-moderate erosive and severe erosive OLP,serum miR-155-5p levels,Th17 ratio and Th17/Treg in peripheral blood gradually increased,while serum SOCS1 mRNA level and Treg ratio in peripheral blood gradually decreased(all P＜0.05).Correlation analysis showed that the serum miR-155-5p level was negatively correlated with serum SOCS1 mRNA level in OLP patients(r=-0.809,P＜0.01).Serum miR-155-5p level was positively correlated with Th17 ratio and Th17/Treg in peripheral blood(r/rs=0.819 and 0.820,both P＜0.05),and was negatively correlated with Treg ratio(r=-0.735,P＜0.05).Serum SOCS1 mRNA level was nega-tively correlated with Th17 ratio and Th17/Treg in peripheral blood(r/rs=-0.770 and-0.790,respectively;both P＜0.05),and was positively correlated with Treg ratio(r=0.733,P＜0.05).Conclusion The serum miR-155-5p level increased and serum SOCS1 mRNA level decreased in OLP patients,and the changes of both levels were closely related to the severity of disease and Th17/Treg imbalance.关键词
口腔扁平苔藓/微小核糖核酸-155-5p/细胞因子信号传导抑制蛋白1/病损程度/辅助性T细胞17/调节性T细胞失衡Key words
oral lichen planus/microRNA-155-5p/suppressor of cytokine signaling1/degree of lesion/helper T cell 17/regulatory T cell imbalance分类
医药卫生引用本文复制引用
李玲玲,梁伟腾,后珉红..口腔扁平苔藓患者血清miR-155-5p、SOCS1 mRNA水平与病损程度及Th17/Treg失衡的关系[J].山东医药,2024,64(13):26-29,35,5.基金项目
天津市卫生健康科技项目(19ZXMFCY0081). （19ZXMFCY0081）
