生物技术通报2024，Vol.40Issue(4)：52-66,15.DOI:10.13560/j.cnki.biotech.bull.1985.2023-1044
单病毒示踪技术在动植物细胞中的应用进展
Application of Single-virus Tracking Technique in Animal and Plant Cells
摘要
Abstract
Virus is a kind of simple microorganism specializing in intracellular parasitics,which is one of the main pathogens causing animal and plant diseases.The development of single-virus tracking(SVT)technology makes it possible to reveal the fine process of viral life activity.SVT is a new method to study the mechanism of viral infection based on fluorescence labeling and high spatio-temporal resolution imaging,which allows the observation of the life activity of a single or multiple viruses.In recent years,the SVT technology has made great progress in the study of virus entry,replication,and intercellular transmission,providing new insights into the mechanism of virus infection.In this paper,the principle and strategy types of virus labeling are briefly introduced,and the research progress of virus fluorescence labeling in single virus tracing is summarized,especially the labeling and imaging methods of different structures of viruses,aiming to provide new ideas for optimizing labeling strategies and solving the problem of efficient labeling of different types and structures of viruses.关键词
病毒标记/单病毒示踪/荧光染料/荧光蛋白/量子点Key words
virus labeling/single virus tracking/fluorescent dye/fluorescent protein/quantum dots引用本文复制引用
袁笑妍,张御格,姚丽娟,唐晨,李晓娟..单病毒示踪技术在动植物细胞中的应用进展[J].生物技术通报,2024,40(4):52-66,15.基金项目
第三次新疆综合科考项目(2022xjkk1200),国家自然科学基金项目(91954202,31871349),北京林业大学科技创新计划项目(2019J003003) （2022xjkk1200）
