生物技术通报2024，Vol.40Issue(4)：110-121,12.DOI:10.13560/j.cnki.biotech.bull.1985.2023-1102
大豆GmHMGS基因的鉴定及表达模式分析
Identification and Expression Pattern Analysis of GmHMGS Gene in Soybean
摘要
Abstract
[Objective]3-hydroxy-3-methylglutaryl-CoA synthase(HMGS)is a key enzyme in the mevalonate pathway and plays an important role in the growth,development and synthesis of terpenoids.Bioinformatics study and expression pattern analysis of the GmHMGS gene can lay the foundation for exploring the function of GmHMGS gene.[Method]The gene was identified and analysed using bioinformatics approaches,and then its expression pattern was examined using real-time fluorescence quantitative PCR(RT-qPCR).[Result]The GmHMGS gene had six family members in total,named GmHMGS1 to GmHMG6.The analysis of physicochemical properties and gene structure showed that GmHMGS3,GmHMG4,GmHMGS5,GmHMGS6 were stable hydrophilic proteins,and all GmHMGS possessed conserved functional domains of HMG-CoA synthase.Promoter sequence analysis revealed that GmHMGS has hormone and adversity stress related action elements.The analysis of tissue expression pattern revealed that GmHMGS was expressed in roots,stems,leaves,flowers,grains,pod skins,and root nodules,with leaves having the highest relative expression of GmHMGS1,GmHMG2,GmHMG3,GmHMG5,and GmHMG6,and roots having the highest relative expression of GmHMGS4.The GmHMGS genes were responsive to PEG6000,NaCl,and H2O2 stresses,as well as MeJA and ABA exogenous hormones,and the relative expression of GmHMGS1 and GmHMGS6 was higher under a wide range of adversity stresses,according to expression analysis under abiotic stress and different exogenous hormone-induced conditions.[Conclusion]GmHMGS gene family may be involved in response to drought,oxidative and salt stresses in soybean.关键词
大豆/GmHMGS/生信分析/表达分析/胁迫处理Key words
soybean/GmHMGS/biocredit analysis/expression analysis/stress treatment引用本文复制引用
娄银,高浩竣,王茜,牛景萍,王敏,杜维俊,岳爱琴..大豆GmHMGS基因的鉴定及表达模式分析[J].生物技术通报,2024,40(4):110-121,12.基金项目
山西省科技重大专项计划揭榜挂帅项目子课题(202201140601025-3-06),山西省科技成果转化引导专项计划(202104021301038),中央引导地方科技发展资金项目(YDZJSX2022A035),山西农业大学科技创新基金项目(2018YJ29),山西省研究生教育创新项目(2023SJ106) （202201140601025-3-06）
