生物技术通报2024，Vol.40Issue(4)：122-129,8.DOI:10.13560/j.cnki.biotech.bull.1985.2023-1082
大白菜BrMLP328的克隆、表达及功能验证
Cloning,Expression and Functional Identification of BrMLP328 Gene in Brassica rapa subsp.pekinensis
摘要
Abstract
[Objective]The BrMLP328 gene of Chinese cabbage(Brassica rapa subsp.pekinensis)was cloned,its expression pattern was analysed,and its function in regulating flowering was verified.This may lay the basis for further investigation into the mechanism of the role of this gene in regulating flower formation in Chinese cabbage.[Method]BrMLP328 was cloned by RT-PCR and analysed by bioinformatics.RT-qPCR was used to determine the relative expression of the BrMLP328.An overexpression vector was constructed and transformed into wild-type Arabidopsis thaliana by flower-dipping method,and the difference in flowering time between the T2 generation plants and the wild type was compared.[Result]The coding sequence(CDS)of BrMLP328 has a total length of 456 bp,encoding 151 amino acids.The relative molecular weight of the protein was 17 493.82 Da and was localized in the nucleus.The expression of BrMLP328 was the highest in the stems of Chinese cabbage,followed by roots and lowest in flower buds.The expression in the growing point of the stem tip showed an elevated level after vernalization,after which it declined rapidly at the stage of flower bud differentiation and was maintained at a very low level.Compared with the wild type,the flowering time of the BrMLP328 of T2 generation of transgenic plants delayed by 1.46-3.09 d.[Conclusion]BrMLP328 was cloned from Chinese cabbage.The expression of BrMLP328 was different in different tissues and different flowering stages,and the gene could delay flowering.关键词
大白菜/BrMLP328/成花转变/基因克隆/功能验证Key words
Brassica rapa subsp.pekinensis/BrMLP328/flowering transformation/gene cloning/functional verification引用本文复制引用
杜泽光,任少文,张凤勤,李梅兰,李改珍,齐仙惠..大白菜BrMLP328的克隆、表达及功能验证[J].生物技术通报,2024,40(4):122-129,8.基金项目
山西省基础研究计划项目(20210302124244),山西省重点研发计划项目(202202140601005),山西农业大学生物育种工程项目(YZGC114,YZGC121),国家大宗蔬菜产业技术体系太原综合试验站(CARS-23-G09),大白菜种质资源创新与利用山西省科技创新重点团队(2014131016) （20210302124244）
