生物技术通报2024，Vol.40Issue(4)：139-147,9.DOI:10.13560/j.cnki.biotech.bull.1985.2023-1099
褪黑素对薄皮甜瓜采后软化和乙烯合成的影响
Effects of Melatonin on the Fruit Softening and Ethylene Synthesis of Post-harvest Oriental Melon
摘要
Abstract
[Objective]Melatonin plays an important role in post-harvest fruit preservation.To explore the efects of melatonin on the fruit softening and ethylene synthesis of post-harvest oriental melon.[Method]The fruit(Cucumis melo cv.'Huaguniang')were soaked at 0,100,200,300,400 μmol/L melatonin solution,by measuring the firmness,weight loss,decay rate,and endogenous ethylene production,the optimal concentration was selected.At the optimal concentration,the ACC content and the activities of ACO and ACS were studied.Furthermore,the transcriptome sequecing and real-time PCR were used to compare the gene expression of ethylene synthesis,signal transduction pathway and fruit softening between melatonin treatment and control.[Result]200 μmol/L treatment delayed the weight loss rate,slow down firmness,and prevent fruit decay and deterioration effectively.Melatonin treatment decreased the ethylene synthesis of fruit and ACC level,while did not delay the arrival of peak period.The transcriptome sequecing results indicated that there were differentially expressed genes in ethylene synthesis,signal transduction pathways and fruit softening between melatonin and control fruits.Melatonin treatment inhibited the expressions of CmACO1-2,CmACS1,CmERFs,CmEXPs and CmEFRs.[Conclusion]200 μmol/L melatonin delays the weight loss rate,slows down firmness,and decreases the ethylene synthesis,by inhibiting the expressions of genes in ethylene synthesis,signal transduction pathways and fruit softening and prolong the storaage life.关键词
薄皮甜瓜/褪黑素处理/乙烯合成/果实软化/基因表达Key words
oriental melon/melatonin treatment/ethylene synthesis/fruit softening/gene expression引用本文复制引用
陈强,黄馨慧,张峥,张冲,柳叶飞..褪黑素对薄皮甜瓜采后软化和乙烯合成的影响[J].生物技术通报,2024,40(4):139-147,9.基金项目
辽宁省教育厅项目(LJKMZ20221467),国家大学生创新创业训练项目(202310166014),沈阳师范大学博士科研启动基金(BS202106) （LJKMZ20221467）
