生物技术通报2024，Vol.40Issue(4)：148-158,11.DOI:10.13560/j.cnki.biotech.bull.1985.2023-1045
盐胁迫下辣椒CaPIF4的表达特性与功能分析
Expression Characteristics and Functions of CaPIF4 in Capsicum annuum Under Salt Stress
摘要
Abstract
[Objective]Studying the molecular mechanisms of the pepper photoreceptor pigment phytochrome-interacting factor 4(PIF4)in response to salt stress plays an important role in understanding the molecular mechanisms of pepper's response to salt stress and breeding salt-tolerant varieties.[Method]Using'Zunla-1'cDNA as a template,CaPIF4 was cloned.Then the physical and chemical properties of CaPIF4 were analyzed using bioinformatics software.And the expression pattern and salt stress-induced expression was studied by real-time fluorescence quantitative PCR(RT-qPCR)and virus-induced gene silencing technology(VIGS).[Result]CaPIF4 was expressed in various tissues of pepper,including roots,stems,leaves,flowers,and fruits,with the highest expression in the leaves.And the expression of CaPIF4 increased with the extension of 300 mmol/L NaCl treatment,and reached the highest at 2 h after treatment.CaPIF4 gene was silenced using VIGS technology.The control group(CK)and gene transient silencing group(pTRV-CaPIF4)were treated in 300 mmol/L NaCl.And the phenotype showed that pTRV-CaPIF4 plants after salt stress presented more obvious salty damage compared with CK.Using VIGS technology to silence CaPIF4,the CK and the pTRV-CaPIF4 were treated with 300 mmol/L NaCl.It was found that the plants in the pTRV-CaPIF4 group were more severely wilted compared to CK after gene silencing,indicating a decrease in salt tolerance in the pTRV-CaPIF4 group.Subcellular localization results showed that CaPIF4 was located in the nucleus.Yeast transcriptional activity analysis revealed that CaPIF4 had transcriptional activation activity.Under high salt stress,the hydrogen peroxide content and antioxidant enzyme activity in the pepper leaves increased.Staining results showed that silencing the CaPIF4 gene resulted in a significant increase in H2O2 and O2-levels.[Conclusion]CaPIF4,as a transcription factor,may be involved in regulating salt tolerance in peppers.关键词
辣椒/盐胁迫/CaPIF4/转录因子/活性氧Key words
Capsicum annuum/salt stress/CaPIF4/transcription factor/ROS引用本文复制引用
李慧,杨有新,刘建萍,文钰芳,王悦,纪超,石国优,罗英,周勇,李志敏,吴晓玉..盐胁迫下辣椒CaPIF4的表达特性与功能分析[J].生物技术通报,2024,40(4):148-158,11.基金项目
江西省主要学科学术和技术带头人培养计划,青年人才项目(20204BCJL23044),国家自然科学基金项目(32060047),江西省自然科学基金面上项目(20232BAB205041),赣鄱俊才支持计划,高层次和急需紧缺海外人才引进项目(20232BCJ25068) （20204BCJL23044）
