生物技术通报2024，Vol.40Issue(4)：167-178,12.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0970
西洋参bZIP基因家族全基因组鉴定和表达分析
Genome Wide Identification and Expression Analysis of the bZIP Gene Family in Panax quinquefolius
摘要
Abstract
[Objective]The basic leucine zipper(bZIP)is one of the largest transcription factor families in plants,involved in multiple processes such as plant growth and hormone regulation.This study aims to identify the bZIP gene family in Panax quinquefolius and analyze its structure and expression patterns,providing a theoretical basis for further research on the function of the bZIP gene family in P.quinquefolius.[Method]Bioinformatics was used to analyze the physicochemical properties,phylogenetic relationships,gene structure,conserved motifs,cis acting elements,chromosomal localization,evolutionary selection pressure,and tissue-specific expression of the bZIP gene in P.quinquefolius.Simultaneously,exogenous hormone ABA was used to treat P.quinquefolius for transcriptome data analysis and RT-qPCR.[Result]A total of 121 bZIPs genes were identified in P.quinquefolius.According to the phylogenetic tree,these genes were divided into 12 subgroups.Gene structure and conserved motif analysis showed that proteins in the same subgroup had similar conserved motifs and domains,with conserved motif 1 being the most widely distributed and highly conserved in the bZIP protein of P.quinquefolius.The analysis of cis-acting elements showed that there were hormone responsive elements,defense and stress responsive elements in the upstream promoter element of bZIP,with ABA hormone responsive elements accounting for the largest proportion.Chromosome localization analysis showed that bZIP genes were unevenly distributed on 24 chromosomes.Conducting evolutionary selection pressure analysis,it was found that purification selection played an important role in the evolution of the bZIP gene family in P.quinquefolius;organizational specific expression analysis showed that the expression levels of PqbZIPs genes were relatively high in flowers and roots;transcriptome data revealed that bZIP transcription factors actively responded to ABA hormone treatment.Subsequently,RT-qPCR experiments were conducted to verify that the trend of gene expression changes was basically consistent.[Conclusion]Members of the PqbZIP gene family have tissue-specific expression patterns and most respond to ABA hormone treatment.关键词
bZIP/转录因子/西洋参/ABA/转录组Key words
bZIP/transcription factors/Panax quinquefolius/ABA/transcriptome引用本文复制引用
郭纯,宋桂梅,闫艳,邸鹏,王英平..西洋参bZIP基因家族全基因组鉴定和表达分析[J].生物技术通报,2024,40(4):167-178,12.基金项目
吉林省科技发展计划项目(20210101190JC),国家重点研发计划项目(2021YFD1600900) （20210101190JC）
