生物技术通报2024，Vol.40Issue(4)：217-227,11.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0905
耐盐植物促生菌W-1鉴定及其对红豆草耐盐性的影响
Identification of Salt-tolerant Plant Growth-promoting Bacterium W-1 and Its Effect on the Salt-tolerance of Sainfoin(Onobrychis viciaefolia)
摘要
Abstract
[Objective]To obtain salt-tolerant plant growth-promoting bacteria,several strains of salt-tolerant bacteria were isolated from plant roots in a saline-alkali soil in Jingtai,Gansu province,and some of them had obvious plant growth-promoting characteristics.[Method]Based on the previous work,a strain of salt-tolerant bacterium W-1 and a salt-sensitive plant sainfoin(Onobrychis viciaefolia)were selected as experimental materials,and the species classification,salt tolerance,acid and alkali tolerance and plant growth-promoting characteristics of strain W-1 were tested.The effects of strain W-1 on the physiological status of sainfoin treated with different concentrations of NaCl were investigated.[Result]The salt-tolerant bacterium W-1 was Gram-positive,spore-producing,without capsule and flagellated.The 16S rDNA sequencing and comparison results showed that the bacterium W-1 was Bacillus siamensis.The maximum NaCl tolerance of strain W-1 was 13%,and the pH tolerance range was 4.5-8.5.W-1 has various plant growth-promoting functions,such as phosphate and potassium solubilization,nitrogen fixation,siderophores production,indole-3-acetic acid(IAA)production and 1-aminocyclopropane-1-carboxylic acid(ACC)deaminase activity.Strain W-1 significantly increased dry weight and fresh weight and promoted root growth of sainfoin seedlings.Under 100 and 150 mmol/L NaCl stresses,strain W-1 significantly increased the contents of soluble sugar,soluble protein,proline,chlorophyll,and catalase activity,decreased the content of hydrogen peroxide,leaf yellowing rate and mortality,and alleviated the adverse effects of salt stress on the growth of sainfoin seedlings.[Conclusion]The W-1 strain was an excellent plant growth-promoting bacterium with salt tolerance,acid and alkali tolerance,and enhanced the salt tolerance of legume forage.关键词
植物促生菌/吲哚-3-乙酸/固氮/溶磷/ACC脱氨酶/盐胁迫Key words
plant growth-promoting bacteria/indole-3-acetic acid/nitrogen fixation/phosphate solubilization/ACC-deaminase/salt stress引用本文复制引用
高志伟,魏明,于祖隆,伍国强,魏俊龙..耐盐植物促生菌W-1鉴定及其对红豆草耐盐性的影响[J].生物技术通报,2024,40(4):217-227,11.基金项目
国家自然科学基金项目(32360483,32160466),甘肃省自然科学基金重点项目(23JRRA764) （32360483,32160466）
