生物技术通报2024，Vol.40Issue(4)：228-241,14.DOI:10.13560/j.cnki.biotech.bull.1985.2023-1172
生防细菌HX0037对栝楼炭疽病的防病能力及其机制
Efficacy and Its Mechanism of Bacterial Strain HX0037 on the Control of Anthracnose Disease of Trichosanthes kirilowii Maxim
摘要
Abstract
[Objective]The biocontrol ability of bacterium HX0037 against anthracnose disease of Trichosanthes kirilowii Maxim and its biocontrol mechanism were studied.[Method]The antagonistic activities of HX0037 against four pathogens of fruit anthracnose disease were determined in petri dishes experiments.The biocontrol efficiencies of HX0037 against Trichosanthes leaf anthracnose and Trichosanthes fruit anthracnose were evaluated on detached leaves and fruits in vitro.In addition,HX0037 was further identified based on the analyses on morphological features,physiological and biochemical characteristics and molecular biological identification results of the whole genome,and the secondary metabolites of biocontrol bacteria HX0037 were also predicted.The lipopeptide compounds produced by strain HX0037 were extracted by acid precipitation and analyzed by high-performance liquid chromatography tandem mass spectrometry(LC-MS).Finally,the possible biocontrol mechanism was explored through two experiments,observing the effects of lipopeptides produced by strain HX0037 on the hyphal growth and morphology of Colletotrichum gloeosporioides,and the abilities of strain HX0037 to produce enzyme and siderophore.[Result]Strain HX0037 showed varied antimicrobial activity against four pathogens of fruit anthracnose disease,especially the best against C.gloeosporioides,a pathogen of Trichosanthes anthracnose disease,and it obviously changed the cell membrane permeability of the pathogenic fungi.Compared with plant tissues inoculated with the pathogen alone,inoculation with strain HX0037 significantly reduced the incidence of leaf and fruit anthracnose diseases of T.kirilowii Maxim,and the biocontrol efficacy was 61.50%and 52.51%,respectively.Strain HX0037 was further identified as Bacillus amyloliquefaciens,and its genome contained 12 secondary metabolite gene clusters,and it decomposed the protein,cellulose and starch,and also produced siderophore.The hyphae of C.gloeosporioides were distorted,intumescent,broken and more transparent after treatment with the antifungal lipopeptide produced by strain HX0037.Surfactin and fengycin were detected from the lipopeptide extracts of strain HX0037 by LC-MS.[Conclusion]The B.amyloliquefaciens HX0037 has strong antagonistic activity on C.gloeosporioides,and is expected to be further developed as a biological agent for the biocontrol of Trichosanthes anthracnose diseases.关键词
解淀粉芽孢杆菌/生物防治/栝楼炭疽病/拮抗/全基因组/菌种鉴定/脂肽Key words
Bacillus amyloliquefaciens/biological control/Trichosanthes anthracnose disease/antagonism/whole genome/species identification/lipopeptide引用本文复制引用
徐伟芳,李贺宇,张慧,何仔昂,高文恒,谢紫洋,王传文,尹登科..生防细菌HX0037对栝楼炭疽病的防病能力及其机制[J].生物技术通报,2024,40(4):228-241,14.基金项目
安徽省自然科学基金项目(2108085QH374),中药研究与开发安徽省重点实验室开放基金(AKLPDCM202310),大健康研究院安徽中药材种植联合研究中心专项资金(yjzx2023005),安徽中医药大学-潜山市医药健康发展技术研究中心科研攻关研发项目(2021QSHZ01),安徽中医药大学人才支持项目(2020rcyb008),大学生创新创业训练计划省级项目(S202210369005) （2108085QH374）
