生物技术通报2024，Vol.40Issue(4)：297-305,9.DOI:10.13560/j.cnki.biotech.bull.1985.2023-1068
铜绿假单胞菌潜在VI型分泌系统效应蛋白PA0423的鉴定及功能研究
Identification and Functional Study of T6SS Effector Protein PA0423 of Pseudomonas aeruginosa
摘要
Abstract
[Objective]The objective of this study is to investigate the novel effector proteins and their functions in the type VI secretion system(T6SS).[Method]The secretion mode and function of PA0423 were explored by gene knockout,immunoblotting,co-immunoprecipitation,intraspecific and interspecific competition,Escherichia coli toxicity assays and crystal violet biofilm assays.[Result]Western blotting and co-immunoprecipitation experiments showed that PA0423 interacted with PA0262,and the secretion of PA0423 depended on H2-T6SS.PA0423 is a H2-T6SS-dependent antimicrobial effector protein with interspecific competitive advantages.PA0423 is toxic to Escherichia coli and PA0422 is its immune protein.PA0423 affected the formation of bacterial biofilms.[Conclusion]PA0423 is a H2-T6SS-dependent antimicrobial effector protein,which has bactericidal function and affects the formation of bacterial biofilms.关键词
铜绿假单胞菌/VI型分泌系统/PA0423/效应蛋白Key words
Pseudomonas aeruginosa/type VI secretion system/PA0423/effector proteins引用本文复制引用
王彩虹,蒋梦媛,邵煜涵..铜绿假单胞菌潜在VI型分泌系统效应蛋白PA0423的鉴定及功能研究[J].生物技术通报,2024,40(4):297-305,9.基金项目
北京市医管中心青苗项目(QML20230105) （QML20230105）
