铜绿假单胞菌潜在VI型分泌系统效应蛋白PA0423的鉴定及功能研究OA北大核心CSTPCD
Identification and Functional Study of T6SS Effector Protein PA0423 of Pseudomonas aeruginosa
[目的]探讨VI型分泌系统(type VI secretion system,T6SS)新的效应蛋白及其功能.[方法]以铜绿假单胞菌PAO1 为研究对象,通过基因敲除、免疫印迹、免疫共沉淀、种内和种间竞争、大肠杆菌毒性实验以及结晶紫生物膜等实验探索PA0423 的分泌方式和功能.[结果]免疫印迹和免疫共沉淀实验表明PA0423 可以与PA0262 相互作用,且PA0423 的分泌依赖于H2-T6SS;竞争实验表明PA0423 是H2-T6SS依赖的抗菌效应蛋白,且具有种间竞争优势;PA0423 具有大肠杆菌毒性且PA0422 为其免疫蛋白;PA0423 能够影响细菌生物膜的形成.[结论]PA0423 为H2-T6SS依赖的抗菌效应蛋白,其具有杀菌功能且影响细菌生物膜的形成.
[Objective]The objective of this study is to investigate the novel effector proteins and their functions in the type VI secretion system(T6SS).[Method]The secretion mode and function of PA0423 were explored by gene knockout,immunoblotting,co-immunoprecipitation,intraspecific and interspecific competition,Escherichia coli toxicity assays and crystal violet biofilm assays.[Result]Western blotting and co-immunoprecipitation experiments showed that PA0423 interacted with PA0262,and the secretion of PA0423 depended on H2-T6SS.PA0423 is a H2-T6SS-dependent antimicrobial effector protein with interspecific competitive advantages.PA0423 is toxic to Escherichia coli and PA0422 is its immune protein.PA0423 affected the formation of bacterial biofilms.[Conclusion]PA0423 is a H2-T6SS-dependent antimicrobial effector protein,which has bactericidal function and affects the formation of bacterial biofilms.
王彩虹;蒋梦媛;邵煜涵
首都医科大学附属北京友谊医院 热带医学研究所,北京 100050
铜绿假单胞菌VI型分泌系统PA0423效应蛋白
Pseudomonas aeruginosatype VI secretion systemPA0423effector proteins
《生物技术通报》 2024 (004)
297-305 / 9
北京市医管中心青苗项目(QML20230105)
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