孤雌卤虫雌激素相关受体基因(ERR)的表达特性分析OA

Estrogen affects the development and maturation of reproductive system of animals through their interactions with estrogen-related receptors.In order to explore the role of estrogen-related receptor gene ERR in ovarian development and maturation of Artemia parthenogenetica,the ERR gene was cloned and analyze by bioinformatics.Moreover,the expres-sion characteristics of this gene in different tissues(ovaries,head and trunk)and gonads at different developmental stages of A.parthenogenetica(early oocytes(EO),late oocytes(LO),early embryos(EE)and later embryos(LE)were further stud-ied.The results showed that the ORF length of ERR gene was 1536 bp,encoding 521 amino acids,and the relative molecular weight and isoelectric point of encoded protein were 5.7114×104 and 5.06,respectively.The protein had two domains with no signal peptide and transmembrane structure.Irregular volumes(46.97%)and α-helix(40.7%)dominated the secondary structure and the tertiary structure corresponded to the secondary structure.In the NJ phylogenetic tree,A.parthenogenetica was found to be most closely related to Daphnia magna and Diaphanosoma celebensis,but was distant from Zootermopsis nevadensis and Coptotermes formosanus.Analysis of the expression characteristic showed that the expression level of ERR gene in head tissues was significantly higher than that in ovarian and trunk and with the maturation of A.parthenogenetica ovary,the expression level of ERR gene was up-regulated gradually,reaching the highest level in EE,and then significantly down-regulated in LE(P＜0.01).