天津科技大学学报2024，Vol.39Issue(2)：34-41,8.DOI:10.13364/j.issn.1672-6510.20230069
孤雌卤虫雌激素相关受体基因(ERR)的表达特性分析
Analysis of Expression Characteristics of Estrogen-Related Receptor Gene(ERR)in Artemia parthenogenetica
摘要
Abstract
Estrogen affects the development and maturation of reproductive system of animals through their interactions with estrogen-related receptors.In order to explore the role of estrogen-related receptor gene ERR in ovarian development and maturation of Artemia parthenogenetica,the ERR gene was cloned and analyze by bioinformatics.Moreover,the expres-sion characteristics of this gene in different tissues(ovaries,head and trunk)and gonads at different developmental stages of A.parthenogenetica(early oocytes(EO),late oocytes(LO),early embryos(EE)and later embryos(LE)were further stud-ied.The results showed that the ORF length of ERR gene was 1536 bp,encoding 521 amino acids,and the relative molecular weight and isoelectric point of encoded protein were 5.7114×104 and 5.06,respectively.The protein had two domains with no signal peptide and transmembrane structure.Irregular volumes(46.97%)and α-helix(40.7%)dominated the secondary structure and the tertiary structure corresponded to the secondary structure.In the NJ phylogenetic tree,A.parthenogenetica was found to be most closely related to Daphnia magna and Diaphanosoma celebensis,but was distant from Zootermopsis nevadensis and Coptotermes formosanus.Analysis of the expression characteristic showed that the expression level of ERR gene in head tissues was significantly higher than that in ovarian and trunk and with the maturation of A.parthenogenetica ovary,the expression level of ERR gene was up-regulated gradually,reaching the highest level in EE,and then significantly down-regulated in LE(P＜0.01).关键词
孤雌卤虫/雌激素相关受体/生物信息学特征/基因表达/卵巢发育Key words
Artemia parthenogenetica/estrogen-related receptors/bioinformatics characteristics/gene expression/ovarian development分类
生物科学引用本文复制引用
刘雪,韩学凯,欧阳雪梅,隋丽英..孤雌卤虫雌激素相关受体基因(ERR)的表达特性分析[J].天津科技大学学报,2024,39(2):34-41,8.基金项目
天津市自然科学基金项目(18JCQNJC78500) （18JCQNJC78500）
教育部长江学者和创新团队发展计划资助项目(IRT-17R81) （IRT-17R81）
