武警医学2024，Vol.35Issue(4)：277-285,9.
铜死亡基因LIPT1在脑胶质瘤诊断及预后判断中的价值
Value of cuproptosis gene LIPT1 in diagnosis and prognosis of brain gliomas
摘要
Abstract
Objective To investigate the potential value of the cuproptosis gene LIPT1 in the diagnosis and prognosis of glio-ma.Methods The expression of 10 cuproptosis genes in glioma was mined using TCGA database.The expression of LIPT1 in 18 glio-ma patients was detected by IHC and qRT-PCR.The expression of LIPT1 in HEB,U87 and U251 cells was detected by qRT-PCR.ROC curve was used to predict the diagnostic efficacy of LIPT1 in glioma.Kaplan-Meier survival curve and Cox analysis were used to explore the prognostic value of LIPT1.ssGSEA database was used to evaluate the effect of LIPT1 on immune invasion of glioma.To ex-plore the relationship between LIPT1 and immunological checkpoints,DNA methylation site and DNA mismatch repair genes in glio-mas,fFunctional enrichment of LIPT1-associated genes was performed with GO,KEGG,and GSEA.Animal TFDB3 predicts LIPT1-associated transcription factors.The GSCA database was utilized to predict potential drugs targeting LIPT1 and hub genes.Results LIPT1 expression in glioma was higher than in normal tissues(3.03 vs.2.28,P<0.05).The immune scores of grade Ⅱ,Ⅲ and Ⅳ gliomas were different[(1.47±0.51)vs.(4.94±1.25)vs.(9.88±1.65),P<0.05].The mRNA levels of LIPT1 in HEB,U87 and U251 cells were different[(0.22±0.03)vs.(0.45±0.04)vs.(0.58±0.05),P<0.05].The AUC value of LIPT1 in glioma diagnosis was 0.87.The OS,DSS,and PFI in the high LIPT1 expression group were shorter than those in the low LIPT1 expression group(P<0.05).The LIPT1 expression was correlated with immune infiltration changes and the expression of immune check-point,DNA methylation site and DNA mismatch repair gene(P<0.05).Transcription factors such as FOXG1 were predicted to possi-bly bind to LIPT1.Drugs such as docetaxel might treat gliomas by tar-geting and regulating LIPT1-related hub genes.Conclusions LIPT1 may be a potential biomarker and may be associated with poor prog-nosis of glioma.关键词
胶质瘤/铜死亡基因脂肪酸转移酶-1/铜死亡/生物标志物Key words
glioma/lipoyltransferase 1/cuproptosis/biomarker分类
医药卫生引用本文复制引用
林彦琛,徐葳,英草,刘瑞,张玉钰,代二庆,李晶晶..铜死亡基因LIPT1在脑胶质瘤诊断及预后判断中的价值[J].武警医学,2024,35(4):277-285,9.基金项目
天津市教委科研计划项目(2022YGYB07) （2022YGYB07）
天津市卫生健康科技项目(TJWJ2022QN100) （TJWJ2022QN100）
