西北植物学报2024，Vol.44Issue(3)：396-407,12.DOI:10.7606/j.issn.1000-4025.20230560
枸杞SWEET基因家族鉴定及其与可溶性糖含量关系
Identification of the SWEET gene family and its relationship with soluble sugar content in wolfberry
摘要
Abstract
[Objective]Sugars will eventually be exported transporters(SWEETs)play an important role in growth and development of plants.The aims of the study were to elucidate the role of the SWEET gene family in sugar accumulation during the development of wolfberry,and to provide a reference for revealing the role of the SWEET genes in the development of wolfberry fruits.[Methods]In the present study,based on genomic data of wolfberry,LbaSWEETs were identified by bioinformatics method,and the ex-pression of LbaSWEETs during fruit development was analyzed using published transcriptional data.[Results]There were 37 members of the SWEET gene family,which were randomly distributed on 10 chromosomes,encoding 152-621 amino acids.The molecular weight of the proteins was 16.87-69.97 kD,and the isoelectric point was 4.96-9.86.Subcellular localizations were predicted to be located in the chloroplast or plasma membrane,most containing seven transmembrane helices.Phylogenetic analysis showed that 37 LbaSWEET proteins were divided into 4 subgroups,which had similar gene structures and conserved motifs.Analysis of promoter elements showed that LbaSWEET promoters were rich in hor-mone response,stress response,and growth response elements.Transcriptome and qRT-PCR analysis showed that the expression of LbaSWEET9 and LbaSWEET29 genes was increased significantly with fruit ripening.Pearson correlation analysis showed that the expression levels of LbaSWEET9 and Lba-SWEET29 genes were significantly positively correlated with fructose content.[Conclusion]LbaSWEET9 and LbaSWEET29 are the key genes that may play an important role in the accumulation of fructose sugar.关键词
枸杞/SWEET基因家族/进化分析/表达分析/可溶性糖含量Key words
wolfberry/SWEET gene family/evolutionary analysis/expression analysis/soluble sugar content分类
农业科技引用本文复制引用
尹跃,秦小雅,赵建华,杨萍,何军,安巍..枸杞SWEET基因家族鉴定及其与可溶性糖含量关系[J].西北植物学报,2024,44(3):396-407,12.基金项目
宁夏自然科学基金优秀青年基金项目(2023AAC05049) （2023AAC05049）
宁夏回族自治区重点研发项目(2022BBF02008) （2022BBF02008）
