西北植物学报2024，Vol.44Issue(3)：408-420,13.DOI:10.7606/j.issn.1000-4025.20230546
普通丝瓜PYL基因家族鉴定及其在果实发育中的表达分析
Genome-wide identification of the PYL gene family in Luff a cylindrica and expression analysis in fruit development
摘要
Abstract
[Objective]Expression of PYL genes in Luffa cylindrica under ABA treatment was studied to provide a reference for gene function and to provide foundation for molecular breeding to improve the quali-ty and yield of L.cylindrica.[Methods]Bioinformatics methods were used to analyze the distribution,chromosome location,gene structure,gene collinearity,selection pressure,evolutionary relationships,and cis-acting elements of the PYL gene family in L.cylindrica.Expression of the PYL gene family un-der different concentrations of ABA was verified by qRT-PCR.[Results]Eleven LcPYL genes were identi-fied in the genome of L.cylindrica.LcPYL genes had CDSs of 576-966 bp with typical PYRPYLRCAR_like structures.Collinear analysis identified seven repeating gene pairs that were predicted to have similar functions.LcPYL promoters contained cis-acting elements associated with plant hormones,and the ex-pression of its members was tissue-specific.Expression of LcPYLs in L.cylindrica fruits did not change at 2 mg/mL ABA.However,the expression of LcPYL5,LcPYL7,and LcPYLl1 was significantly in-creased at 4 mg/mL ABA,while LcPYL1 and LcPYL7 were expressed at the day of flowering at 8 mg/mL ABA.[Conclusion]Our findings suggest that LcPYL1,LcPYL5,LcPYL7,and LcPYL11 may be closely linked to endogenous ABA biosynthesis.关键词
普通丝瓜/PYL基因家族/果实发育/外源ABA处理/表达分析Key words
Luffa cylindrica/PYL gene family/fruit development/ABA treatment/expression analysis分类
生物科学引用本文复制引用
王玉倩,彭丽娟,刘建汀,温庆放,钟凤林,朱海生..普通丝瓜PYL基因家族鉴定及其在果实发育中的表达分析[J].西北植物学报,2024,44(3):408-420,13.基金项目
福建省属公益类科研院所基本科研专项(2021R1031001,2022R1031007) （2021R1031001,2022R1031007）
福建省自然科学基金项目(2021J01492) （2021J01492）
福建省农业科学院科技创新团队项目(CXTD2021003-1) （CXTD2021003-1）
国家大宗蔬菜产业体系福州综合试验站项目(CARS-23-G51) （CARS-23-G51）
"5511"协同创新工程项目(XTCXGC2021003) （XTCXGC2021003）
