西北植物学报2024，Vol.44Issue(3)：421-429,9.DOI:10.7606/j.issn.1000-4025.20230340
杂交兰ChCAO基因克隆及其在叶艺品系中的表达特征
Molecular cloning and expression analysis of ChCAO gene in leaf art formation of Cymbidium hybrid
摘要
Abstract
[Objective]Chlorophyllide a oxygenase(CAO)is a key enzyme in the formation of chlorophyll b.Cloning and expression analysis of CAO genes may be helpful in exploring their function in regulating leaf color variation of Cymbidium hybrid.[Methods]ChCAO gene was cloned from leaves of Cymbidium hybrid by RACE and RT-PCR.ChCAO was analyzed for structure characteristics,physicochemical prop-erties,sequence alignment,and phylogenetic relationship.The expression of ChCAO in different tissues and leaves of K21-3 were analyzed by qRT-PCR.Meanwhile,ChCAO expression was silenced by VIGS technology.[Results]The results showed that ChCAO coding region was 1 608 bp,encoding 535 amino acids.ChCAO was closely related to Cymbidium sinense,which belonged to genus Cymbidium.qRT-PCR assay showed that the expression of ChCAO was the highest in leaves and the lowest in roots.The expres-sion of ChCAO in K21 leaves was significantly higher than that of K21-3.The VIGS silencing vector of ChCAO gene was constructed and transferred to tobacco.The viral vector was successfully invaded the to-bacco,and the old leaves of tobacco were turned yellow,while chlorophyll content and expression of ChCAO gene were reduced.[Conclusion]The ChCAO gene was obtained and its function was preliminari-ly characterized,which are helpful for the understanding of the formation of leaf art in Cymbidium hybrid.关键词
杂交兰/ChCAO基因/基因克隆/表达分析Key words
Cymbidium hybrid/ChCAO gene/gene cloning/expression analysis分类
农业科技引用本文复制引用
林榕燕,孔兰,吴建设,林兵,钟淮钦..杂交兰ChCAO基因克隆及其在叶艺品系中的表达特征[J].西北植物学报,2024,44(3):421-429,9.基金项目
福建省自然科学基金项目(2023J01199) （2023J01199）
福建省农业科学院英才项目(YC2021013) （YC2021013）
福建省农业科学院科技创新平台专项(CX-PT202102) （CX-PT202102）
福建省农业科学院创新团队建设项目(CXTD2021010-2) （CXTD2021010-2）
