西北植物学报2024，Vol.44Issue(4)：529-538,10.DOI:10.7606/j.issn.1000-4025.20230574
外源茉莉酸甲酯诱导大麦叶斑病抗性研究
Induction of resistance to spot blotch in Hordeum vulgare L.by exogenous methyl jasmonate
摘要
Abstract
[Objective]The study aims to investigate the differential efficacy of anti-leaf spot in barley(Hordeum vulgare L.)of various concentrations of exogenous methyl jasmonate(MeJA)and understand the underlying mechanisms,thereby providing a foundation for the application of MeJA in controlling bar-ley leaf spot.[Methods]We used'Mengpimai 3'(MP3)seedlings as materials and conducted three differ-ent treatments:Inoculating the barley leaves with sterile water without any Bipolaris sorokiniana(the causal agent of the disease),inoculating the barley leaves with sterile water after treatment,and treating the barley leaves with different concentrations(0.5,1.0,1.5,2.0,2.5 mmol/L)of methyl jasmonate(MeJA)before inoculation with the pathogen.The disease incidence was investigated at the three-leaf stage based on disease index to determine the optimal MeJA concentration.We measured antioxidant en-zyme activities,anti-disease-related enzyme activities,malondialdehyde,proline,soluble sugar,and solu-ble protein contents,and the expression levels of related genes under no inoculating treatment,inoculation treatment,and inoculating+optimal MeJA concentration treatment.[Results](1)Exogenous application of MeJA enhanced MP3 resistance against spot blotch,particularly at 1.5 mmol/L where there was a sig-nificant reduction of 19.03％(P＜0.05)in disease index compared with control plants,indicating the most effective induction of resistance.(2)In comparison with inoculating treatment,barley leaves treated with 1.5 mmol/L MeJA exhibited significantly increased activities of superoxide,peroxidase,catalase,chitinase,and β-1,3-glucanas.Additionally,there was a significant decrease in malondialdehyde content as well as proline,soluble sugar,and soluble protein levels.Furthermore,the expression of MeJA-regulated transcription factors and genes encoding disease resistance-related enzymes were significantly upregulated.[Conclusion]Exogenous application of 1.5 mmol/L MeJA enhanced resistance against spot blotch in barley by regulating the activity of disease-related enzymes and the content of osmotic regulatory substances,as well as modulating the expression of genes encoding the disease-resistant enzymes and key transcription factors involved in jasmonic acid signaling pathway.关键词
大麦/茉莉酸甲酯/叶斑病/诱导抗性/抗氧化酶Key words
Hordeum vulgare L./methyl jasmonate/spot blotch/induced resistance/defense enzymes分类
农业科技引用本文复制引用
叶霈颖,司二静,鲁宗辉,汪军成,王化俊,孟亚雄..外源茉莉酸甲酯诱导大麦叶斑病抗性研究[J].西北植物学报,2024,44(4):529-538,10.基金项目
国家自然科学基金项目(31960426,32160496) （31960426,32160496）
国家大麦青稞产业技术体系项目(CARS-05-03B-03) （CARS-05-03B-03）
