西北植物学报2024，Vol.44Issue(4)：572-581,10.DOI:10.7606/j.issn.1000-4025.20230590
茶树NAC转录因子基因CsNAC79和CsNAC9鉴定及其对非生物胁迫的响应
Identification of NAC transcription factor genes CsNAC79 and CsNAC9 in tea plant and their response to different abiotic stresses
摘要
Abstract
[Objective]The study aims to identify the NAC transcription factor genes CsNAC79 and Cs-NAC9 in tea plants and analyze their expression patterns under drought,salt,high temperature,and low temperature stresses as well as ABA and GA3 treatments in order to provide references for functional anal-ysis of CsNAC79 and CsNAC9.[Methods]CsNAC79 and CsNAC9 were amplified by RT-PCR from tea plant'Longjing 43'and analyzed by bioinformatics.The expression of CsNAC79 and CsNAC9 under abi-otic stresses in tea plants was analyzed by qRT-PCR.[Results](1)CsNAC79 and CsNAC9 encoded 409 and 282 amino acids,respectively,and contain the conserved NAM domain of the NAC family at 15-150 amino acids and 11-134 amino acids,respectively.(2)CsNAC79 protein was closely related to Actinidia chinensis,Diospyros kaki,and Cynara scolymus,while CsNAC9 protein was closely related to Amygda-lus persica,Litchi chinensis,and Durio zibethinus.Both of the NAC transcription factors were hydro-philic proteins without signal peptides and transmembrane structures.The promoter regions of CsNAC79 and CssNAC9 contained abiotic stress-responsive elements.Protein structure analysis showed that Cs-NAC79 and CsNAC9 proteins were mainly composed of random coils and a-helixes.(3)Expression analy-sis showed that CsNAC79 and CsNAC9 genes were up-regulated under different abiotic stresses.[Conclusion]The CsNAC79 and CsNAC9 genes in tea plants responded to various abiotic stresses.关键词
茶树/NAC转录因子/ABA/GA3/非生物胁迫/表达分析Key words
tea plants/NAC transcription factor/ABA/GA3/abiotic stress/expression analysis分类
农业科技引用本文复制引用
孔洁玙,杨妮,罗微,胡志航,王雅慧,庄静..茶树NAC转录因子基因CsNAC79和CsNAC9鉴定及其对非生物胁迫的响应[J].西北植物学报,2024,44(4):572-581,10.基金项目
国家自然科学基金项目(31870681) （31870681）
江苏省政策引导类计划项目(SZ-LYG202126) （SZ-LYG202126）
江苏高校优势学科建设项目(PAPD) （PAPD）
