现代科学仪器2024，Vol.41Issue(2)：25-30,6.
基于糖化酶的布鲁氏菌抗体快速检测方法的建立与评估
Establishment and evaluation of a rapid detection method for brucella antibodies based on glucoamylase
蒋小丽1
作者信息
- 1. 简阳市人民医院实验医学科,四川成都 641400
- 折叠
摘要
Abstract
Objective:To Establish a new method for relative quantitative detection of Brucella antibodies and evaluate its performance.Method:Verify liquefaction conditions;Establish the standard curve of the reaction value between glucoamylase and Glucose meter;Preparation of enzyme-linked antibodies and ELISA plates for sample detection and validation of reaction system interference.Results:The concentration of glucoamylase is in direct proportion to the concentration of sugar,and the glucoamylase Brucella Lipopolysaccharide antigen coupling-1 has better discrimination between yin and yang.The interference verification test found that the levels of glucose,HBsAg,HBsAb,HCV and BSA in clinical samples have no impact on the detection results.Conclusion:By constructing an ELISA based on glucose detection and using a convenient Glucose meter,we can quickly detect brucella antibodies,providing a new means for clinical diagnosis.关键词
布鲁氏菌/糖化酶/抗体检测/诊断Key words
Key Wrods Brucella/glucoamylase/antibody detection/diagnosis引用本文复制引用
蒋小丽..基于糖化酶的布鲁氏菌抗体快速检测方法的建立与评估[J].现代科学仪器,2024,41(2):25-30,6.
