西南林业大学学报2024，Vol.44Issue(3)：20-26,7.DOI:10.11929/j.swfu.202303040
葡萄风信子愈伤组织再生体系的构建
Construction of Callus Regeneration System in Grape Hyacinth
摘要
Abstract
The callus induction and plant regeneration of grape hyacinth was carried out,utilizing the flower buds and leaves of Muscari armeniacum as explants.The results show that the best medium for callus induction and proliferation was MS+1.0 mg/L 2,4-D+0.1 mg/L 6-BA,and the callus induction rate was 100％.After 35 days of subculture,there was no significant difference on callus proliferation in different medium formulations.Meanwhile,liquid culture and light culture were beneficial to the proliferation of calli.After 4 cycles of the sub-culture of 1 g M.armeniacum flower and leaf-derived callus,the maximum average fresh weight was 10.36±1.13 g and 10.55±2.29 g.The results showed that different media formulations had no significant effect on flower and leaf-derived callus proliferation in M.armeniacum,and both liquid culture and light culture were beneficial to cal-lus proliferation.Furthermore,embryogenic and non-embryogenic callus could be regenerated to plants by somat-ic embryogenesis and organogenesis,respectively.The regeneration rates were all 100％.关键词
葡萄风信子/愈伤组织/液体培养/器官发生/体细胞胚发生Key words
grape hyacinth/callus/liquid culture/organogenesis/somatic embryogenesis分类
农业科技引用本文复制引用
曹晓云,田淑婷,时春莹,谢靖雯,黎敏婕,杜灵娟..葡萄风信子愈伤组织再生体系的构建[J].西南林业大学学报,2024,44(3):20-26,7.基金项目
国家自然科学基金项目(32171863,31700625)资助. （32171863,31700625）
