西南林业大学学报2024，Vol.44Issue(3)：163-173,11.DOI:10.11929/j.swfu.202306008
羧甲基壳聚糖诱导葡萄柚果实抗病防御代谢物差异分析
Analysis of Resistance and Defense Metabolites of Postharvest Citrus paradisi Induced by Carboxymethyl-chitosan
摘要
Abstract
In order to study the difference of Carboxymethyl-chitosan(CMCS)-induced resistant metabolites inpostharvestgrapefruitfruits.Inthisstudy,grapefruitfruitswereusedastestmaterials,andgrapefruitwasinducedbyl.5％CMCS and sterile water(CK)for 24 h,respectively.The differences of metabolites in grapefruit fruits were ana-lyzed by non-targeted metabolomics.The results showed that a total of 92 differential metabolites were obtained after CMCS induced grapefruit for 24 h,of which 36 were up-regulated and 56 were down-regulated.The KEGG pathway annotation results showed that the differential metabolites were significantly(P＜0.05)enriched into 21 pathways,mainly involved in amino acid and phenylpropanoid metabolism.Further screening found that the ex-pression levels of L-Glutamine,L-Glutamic acid,L-Asparagine,Arginine,Tyramine,Malonic acid,Shikimic acid,D-erythro-3-Methylmalate,trans-Cinnamate and Coumarin changed significantly,and were significantly(P＜0.05)higher than CK.In addition,the related differential metabolites and key resistance differential genes(HSP17.4B,NCED1,GGCT2;2 HSP22.0,WRKY53,HSP22.0,WRKY40,WRKY54)confirmed that there was a significant positive correlation between the two.关键词
羧甲基壳聚糖/代谢组/采后防治/氨基酸代谢/苯丙烷代谢Key words
Carboxymethyl-chitosan/metabolome/prevention and control after mining/amino acid metabol-ism/phenylpropane metabolism分类
农业科技引用本文复制引用
刘胜红,杨清,吴化雨,唐勇,王芳,李根前,邓佳..羧甲基壳聚糖诱导葡萄柚果实抗病防御代谢物差异分析[J].西南林业大学学报,2024,44(3):163-173,11.基金项目
国家自然科学基金项目(31960326、32160394)资助 （31960326、32160394）
云南省"高层次人才培养支持计划"项目(YNWR-QNBJ-2020-205)资助 （YNWR-QNBJ-2020-205）
云南省"两类"人才培养项目(202105AC160045)资助 （202105AC160045）
云南省农业联合专项(202101BD070001-065)资助. （202101BD070001-065）
