心脑血管病防治2024，Vol.24Issue(2)：16-20,封3,6.DOI:10.3969/j.issn.1009-816x.2024.02.004
丹皮酚通过调节p38丝裂原活化蛋白激酶对缺血性脑卒中大鼠神经炎症的影响
Influence of Paeonol on neuroinflammation in ischemic stroke rats by regulating p38 mitogen-activated protein kinase signaling pathway
摘要
Abstract
Objective To investigate the influence of Paeonol on neuroinflammation in ischemic stroke rats and its mechanism.Methods Male SD rats with SPF level were used to establish a rat model of ischemic stroke.Rats were devided into Sham group,Model group,Paeonol group(20 mg/kg Paeonol),and Paeonol combined with p38 mitogen-activated protein kinase(p38 MAPK)activator group(Paeonol+Anisomycin group,20 mg/kg Paeonol+2 mg/kg Anisomycin).After modeling for 48 hours,neurological function deficit score were observed,cerebral infarction volume was detected,neuronal apoptosis and microglia activation were assessed by TUNEL staining and immunofluorescence staining.The levels of tumor necrosis factor α(TNF-α),interleukin(IL)-1β and IL-6 in the brain tissue of the ischemic penumbra were detected by enzyme linked immunosorbent assay(ELISA).The expression of p-p38 MAPK and p38 MAPK was measured by Western blot.Results Compared to the Sham group,rats in the Model group had severe neurological damage,white infarct lesions in the brain tissue,increased neurological function deficit score,cerebral infarct volume,neuron apoptosis rate,microglia cell count and levels of TNF-α,IL-1β,IL-6,and p-p38 MAPK protein expression,the differences were statistically significant(all P<0.05).Compared to the Model group,rats in the Paeonol group exhibited reduced neurological function deficit score,cerebral infarct volume,neuron apoptosis rate,microglia cell count and levels of TNF-α,IL-1β and IL-6,and p-p38 MAPK protein expression,the differences were statistically significant(P<0.05).Compared to the Paeonol group,rats in the Paeonol+Anisomycin group showed increased neurological function deficit score and cerebral infarct volume,and the protective effect of Paeonol on ischemic stroke-induced brain injury and inflammatory response could be reversed by Anisomycin(P<0.05).Conclusion Paeonol may alleviate ischemic stroke-induced brain injury by inhibiting the p38 MAPK pathway,suppressing microglia activation and inflammatory response.关键词
丹皮酚/缺血性脑卒中/神经炎症/p38丝裂原活化蛋白激酶信号通路Key words
Paeonol/Ischemic stroke/Neuroinflammation/P38 mitogen-activated protein kinase signaling pathway引用本文复制引用
周华勇,赵玉萍,杨旭,张珊珊,季一飞..丹皮酚通过调节p38丝裂原活化蛋白激酶对缺血性脑卒中大鼠神经炎症的影响[J].心脑血管病防治,2024,24(2):16-20,封3,6.基金项目
四川省南充市科技局科研项目(NSMC20170454) （NSMC20170454）
