人巨细胞病毒微小RNA UL112调控血管内皮细胞mRNA的研究OACSTPCD
Study of human cytomegalovirus microRNA UL112 regulating vascular endothelial cell mRNA
目的 探讨人巨细胞病毒RNA UL112 对人血管内皮细胞mRNA表达的影响.方法 构建人巨细胞病毒微小RNA UL112 腺病毒过表达载体并使其感染人原代脐静脉内皮细胞作为转染组,感染无义空白腺病毒载体的脐静脉内皮细胞为对照组.检测人巨细胞病毒微小RNA UL112 对脐静脉内皮细胞mRNA表达谱的影响.结果 与对照组相比,转染组mRNA存在差异表达基因365个,其中表达基因上调303个,下调62个,差异表达基因富集于丝裂原活化蛋白激酶通路.结论 人巨细胞病毒微小RNA UL112影响脐静脉内皮细胞mRNA的表达.
Objective To investigate the effect of human cytomegalovirus microRNA UL112(HCMV miR-UL112)on human vascular endothelial.Methods A HCMV miR-UL112 adenovirus overexpression vector was constructed and used to infect human primary umbilical vein endothelial cells(HUVECs)as the transfection group,while HUVECs infected with an ineffective adenovirus served as the control group.The effect of HCMV miR-UL112 on mRNA expression profile of HUVECs were detected.Results In the transfection group,there were 365 differentially expressed genes compared to the control group,with 303 upregulated genes and 62 downregulated genes.The differentially expressed genes were enriched in the mitogen-activated protein kinase(MAPK)signaling pathway.Conclusion HCMV miR-UL112 can affect mRNA expression of HUVECs.
沈凯;邵满芬;蔡聪慧
316000 浙江省舟山市妇女儿童医院(舟山市第三人民医院)心血管内科
人巨细胞病毒微小RNA UL112差异表达丝裂原活化蛋白激酶通路
Human cytomegalovirus microRNA-UL112Differential expressiomMitogen-activated protein kinasesignaling pathway
《心脑血管病防治》 2024 (002)
21-23 / 3
浙江省自然科学基金资助项目(2013LY13H020001);舟山科技计划项目(2012C13024)
评论