心脑血管病防治2024，Vol.24Issue(2)：21-23,3.DOI:10.3969/j.issn.1009-816x.2024.02.005
人巨细胞病毒微小RNA UL112调控血管内皮细胞mRNA的研究
Study of human cytomegalovirus microRNA UL112 regulating vascular endothelial cell mRNA
摘要
Abstract
Objective To investigate the effect of human cytomegalovirus microRNA UL112(HCMV miR-UL112)on human vascular endothelial.Methods A HCMV miR-UL112 adenovirus overexpression vector was constructed and used to infect human primary umbilical vein endothelial cells(HUVECs)as the transfection group,while HUVECs infected with an ineffective adenovirus served as the control group.The effect of HCMV miR-UL112 on mRNA expression profile of HUVECs were detected.Results In the transfection group,there were 365 differentially expressed genes compared to the control group,with 303 upregulated genes and 62 downregulated genes.The differentially expressed genes were enriched in the mitogen-activated protein kinase(MAPK)signaling pathway.Conclusion HCMV miR-UL112 can affect mRNA expression of HUVECs.关键词
人巨细胞病毒微小RNA UL112/差异表达/丝裂原活化蛋白激酶通路Key words
Human cytomegalovirus microRNA-UL112/Differential expressiom/Mitogen-activated protein kinasesignaling pathway引用本文复制引用
沈凯,邵满芬,蔡聪慧..人巨细胞病毒微小RNA UL112调控血管内皮细胞mRNA的研究[J].心脑血管病防治,2024,24(2):21-23,3.基金项目
浙江省自然科学基金资助项目(2013LY13H020001) （2013LY13H020001）
舟山科技计划项目(2012C13024) （2012C13024）
