烟台大学学报（自然科学与工程版）2024，Vol.37Issue(2)：163-171,9.DOI:10.13951/j.cnki.37-1213/n..221105
基于16S rRNA测序的1型和2型糖尿病大鼠肠道菌群差异
Difference of Gut Microbiota between Type 1 and Type 2 Diabetes Rats Based on 16S rRNA Gene Sequencing
摘要
Abstract
Difference between T1DM and T2DM rat gut microflora is analyzed through 16S rRNA gene sequencing.Alpha diversity analysis show that the diversity and evenness of intestinal flora in the T1DM and T2DM groups are higher than those in the control group,and Beta diversity analysis show that there are differences and good cluste-ring among different groups.At the phylum level,the gut microbiota of T1DM and T2DM are dominate by Firmi-cutes and Bacteroidetes.The proportion of Firmicutes/Bacteroidetes(F/B)increases in T1DM and T2DM groups and the ratio in T2DM is higher than T1DM.In T1DM group the abundance of Actinobacteria increases and the a-bundance of Anaerofustis decreases significantly compared with the control group.The abundance of Proteobacteria and Oscillospira increase significantly in T2DM rats.T2DM group shows an increased abundance of Oscillospira,while the contrast is not significant in T1DM group.Rhodococcus is the unique genus in T1DM group,Coprobacillus and Roseburia are the unique genera in T2DM rats.The results show that the imbalance of rat gut microflora is closely related to T1DM and T2DM,Which may provide scientific basis for individualized treatment of T1DM and T2DM.关键词
肠道菌群/T1DM/T2DM/16S rRNA测序Key words
gut microbiota/T1DM/T2DM/16S rRNA sequencing分类
生物科学引用本文复制引用
倪妍,滕新栋,徐丽,王绪敏,曲江勇,王丽君,金黎明,邢志凯,刘秀梅..基于16S rRNA测序的1型和2型糖尿病大鼠肠道菌群差异[J].烟台大学学报（自然科学与工程版）,2024,37(2):163-171,9.基金项目
生物技术与资源利用教育部重点实验室(大连民族大学)开放基金(KF2022006) （大连民族大学）
烟台"双百人才项目"(2320004-SM20RC02). （2320004-SM20RC02）
