烟台大学学报（自然科学与工程版）2024，Vol.37Issue(2)：172-179,8.DOI:10.13951/j.cnki.37-1213/n.221014
蓝隐藻PC645两种β亚基的分离及空间结构差异性
Isolation and Spatial Structure Diversity of Two β Subunits of PC645 from Chroomonas placoidea
摘要
Abstract
In order to identify the spatial structure diversity of a new β subunit found in phycocyanin PC645 of Chroomonas placoidea T13,a large-scale of separation procedure of two steps of DEAE sepharose fast flow anion ex-change chromatography is established and by which a β1 subunit and two β2 subunits components are eluted after 6 mol/L urea denaturation of purified PC645 from self-cultured C.placoidea.The absorption spectra results confirm that the three β subunits elutes are all remained in active state.The circular dichroism(CD)shows that the β1 subunit exhibits similar CD features in visible region to the two β2 subunits,but varies both in the far and near ul-traviolet region.This result indicates that the two types of β subunits have the same bilin composition,but the sec-ondary and tertiary spatial structure of proteins are different.The β1 subunit possesses higher proportion of α helix than that of β2.The results above not only furtherly confirm the existence of new β subunit,but also provide a di-rect experimental basis for re-understanding of the subunit composition and assembly of the specific cryptophytic bil-iproteins.关键词
蓝隐藻/PC645/β亚基/圆二色谱/空间结构差异Key words
Chroomonas placoidea/PC645/β subunit/circular dichroism/spatial structure diversity分类
农业科技引用本文复制引用
张昆,王静,王宇涵,李琴,陈敏..蓝隐藻PC645两种β亚基的分离及空间结构差异性[J].烟台大学学报（自然科学与工程版）,2024,37(2):172-179,8.基金项目
山东省自然科学基金资助项目(ZR2018LD009). （ZR2018LD009）
