烟台大学学报（自然科学与工程版）2024，Vol.37Issue(2)：180-187,8.DOI:10.13951/j.cnki.37-1213/n.221016
基于线粒体基因组全序列的鳅科(Cobitidae)鱼类进化关系分析
Molecular Evolution Analysis of Cobitidae Fishes Based on Mitochondrial Genome Sequences
摘要
Abstract
In order to understand the structural characteristics and phylogenetic information of the mitochondrial ge-nome of Cobitidae,the mitochondrial genome of 40 known Cobitidae are compared and analyzed using Bioinformat-ics,and a phylogenetic tree is constructed using Neighbor-Joining method.The results show that:(1)The length of the mitochondrial genome of Cobitidae ranges from 16 553 to 16 937 bp,and the structural characteristics and gene order are consistent with other teleost fishes.(2)The consensus sequence length of the mitochondrial genome of Co-bitidae is 17 483 bp,the number of variable sites is 8039(45.9%),and the overall mean Kimura 2-Parameter dis-tance genetic distance is 0.19.Among the 13 protein coding genes,ND2 is the highest degree of variation(58.9%)and genetic distance(0.28),the lowest degree of variation is12 S rRNA(30.5%),and the concatenated tRNA sequence is the lowest genetic distance(0.07).(3)Cox1,ND5 and Cytb genes are ideal molecular markers for phylogenetic analysis of Cobitidae.The NJ phylogenetic tree showes that Noemacheilinae is the earliest divergent group and in the ancestral position,Botininae and Cobitinae are closely related and sister groups.This study pro-vides a reference for the evolutionary research of Cobitidae and the selection of molecular markers.关键词
鳅科鱼类/线粒体基因组/系统发育/结构特征Key words
Cobitidae fish/mitochondrial genome/phylogeny/structure characteristics分类
生物科学引用本文复制引用
王熙尧,陈生熬,李瑶瑶,刘一萌,隋智海,全先庆,崔东,刘云国..基于线粒体基因组全序列的鳅科(Cobitidae)鱼类进化关系分析[J].烟台大学学报（自然科学与工程版）,2024,37(2):180-187,8.基金项目
国家自然科学基金资助项目(32072979). （32072979）
