烟台大学学报（自然科学与工程版）2024，Vol.37Issue(2)：188-198,11.DOI:10.13951/j.cnki.37-1213/n.221009
双贝糖浆和君药板蓝根的入血成分及抗炎活性成分
Constituents Absorbed into Blood and Anti-inflammatory Active Constituents of Shuang Bei Tang Jiang and Isatidis Radix
摘要
Abstract
In order to study the anti-inflammatory substance basis of"Shuang Bei Tang Jiang",UHPLC-Q-Orbitrap HRMS is used to identify the chemical components and constituents can be absorbed into blood of Shuang Bei Tang Jiang and Isatidis Radix.The results of UHPLC-Q-Orbitrap HRMS show that the extract of Isatidis Radix contains 71 main chemical components,46 among of them could enter the blood,while 66 of the above chemical compo-nents are detected in Shuang Bei Tang Jiang,42 of them could enter the blood.Further,the anti-inflammatory ac-tive ingredients and the mechanism of action of its main drug Isatidis Radix are discussed through network pharma-cology,and the anti-inflammatory activity of the main active components are verified by Griess method combined with MTT method.The results of network pharmacologic analysis showed that the key anti-inflammatory components are acacia,isovitexin and eupatorin.5-hydroxymethylfurfural,cytidine,isovitexin and syringic acid significantly inhibite NO release induced by LPS at 100 μmol/L in RAW 264.7 cells.Isovitexin can be detected in serum sam-ples of Shuang Bei Tang Jiang and Isatidis Radix.The results of anti-inflammatory activity experiment show that iso-vitexin has the strongest inhibitory effect on NO production,and maybe the key anti-inflammatory active component of Shuang Bei Tang Jiang and Isatidis Radix.关键词
双贝糖浆/板蓝根/UHPLC-Q-Orbitrap HRMS/入血成分/网络药理学/抗炎作用Key words
Shuang Bei Tang Jiang/Isatidis Radix/UHPLC-Q-Orbitrap HRMS/constituents absorbed into blood/network pharmacology/anti-inflammatory effect分类
医药卫生引用本文复制引用
徐瑶瑶,金欣,于向涛,高银鹤,赵烽..双贝糖浆和君药板蓝根的入血成分及抗炎活性成分[J].烟台大学学报（自然科学与工程版）,2024,37(2):188-198,11.基金项目
山东省自然科学基金资助项目(ZR2019ZD24). （ZR2019ZD24）
