医药导报2024，Vol.43Issue(5)：679-688,10.DOI:10.3870/j.issn.1004-0781.2024.05.002
拔脓生肌软膏的药效学及皮肤安全性评价
Pharmacological Effects and Skin Safety Evaluation of Banong Shengji Ointment
摘要
Abstract
Objective To investigate the pharmacological effects of Banong Shengji ointment(BNO)in treating bacte-rial skin infections and wounds,to conduct a preliminary evaluation of its skin safety,and to provide a basis for further research and safe application.Methods SPF mice and rats were used to establish a bacterial skin infection model induced by Staphylo-coccus aureus.These were randomly divided into matrix groups,low,medium,and high dose groups of BNO,Longzhu ointment group,and mupirocin ointment group.After applying the corresponding drugs,the therapeutic effects of BNO on bacterial skin in-fections in mice and rats were observed,including wound healing rate,pathological morphology,reverse culture of skin bacteria,and hydroxyproline(HYP)content in skin tissue,as well as interleukin-10(IL-10),tumor necrosis factor-α(TNF-α)levels in rat skin wound tissue.In addition,mice ear swelling and writhing experiments investigated the anti-inflammatory and analgesic effects of BNO.To evaluate the skin safety of BNO,skin irritation and allergy experiments were conducted.Results Compared with the matrix group,BNO treatment significantly promotes wound healing,improves pathological changes in wound tissue,increa-ses the content of HYP in skin tissue,inhibits bacterial growth at the infection site,suppresses inflammation,and reduces the levels of IL-10 and TNF-α(P＜0.05 or P＜0.01).Besides,the high-dose BNO group significantly inhibits ear swelling in mice(P＜0.01),and both medium and high doses of BNO significantly reduce the number of writhes(P＜0.05 or P＜0.01).Moreover,BNO had no irritation to the intact and damaged skin of New Zealand rabbits and no allergic reactions in guinea pigs,it was safe in the external application.Conclusions BNO has a certain effect of anti-inflammation,analgesia and promoting skin wound healing.The preparation meets the safety requirements of traditional Chinese medicine external ointments.关键词
拔脓生肌软膏/抗菌作用/抗炎作用/镇痛作用/创面愈合/安全性Key words
Banong Shengji ointment/Antibacterial effect/Anti-inflammation effect/Analgesia effect/Wound healing/Safety分类
医药卫生引用本文复制引用
范华娜,邹国发,陶曙红,郭丽冰,尹永芹,陈景胜,陈艳芬..拔脓生肌软膏的药效学及皮肤安全性评价[J].医药导报,2024,43(5):679-688,10.基金项目
广东省公益研究与能力建设专项资金资助项目(2015A030302088) （2015A030302088）
广东省科技厅疾病防治项目(2016A020215158) （2016A020215158）
广东省科技厅-广东省中医药科学院联合项目(2014A020221095) （2014A020221095）
广东省科技计划项目(2014A020212501). （2014A020212501）
