湖北医药学院学报2024，Vol.43Issue(2)：179-184,6.DOI:10.13819/j.issn.2096-708X.2024.02.012
星点设计-响应面法优化毛连菜总有机酸提取工艺
Optimization of Extraction Technology of Total Organic Acids from Picris hieracioides L.by Central Composite Design-Response Surface Method
摘要
Abstract
Objective To optimize the extraction process of total organic acids from Picris hieracioides L..Methods Based on single factor test,the extraction process of total organic acids from Picris hieracioides L.was optimized by using central composite design-response surface method,with liquid-solid ratio,extraction time and ethanol volume fraction as inde-pendent variables,and extraction yield of total organic acids as dependent variable.Results The optimum ultrasonic extrac-tion process of total organic acids from Picris hieracioides L.was as follows:liquid material ratio 29 ∶ 1(mL·g-1),ethanol volume fraction 67%,and extraction time of 43 min.The verification experiment yielded a total organic acid extraction rate of(1.65±0.02)%(n=3,RSD=0.92%),which is close to the predicted value by the model.Conclusion The optimized extraction technology is simple,the process parameters are reliable,and the extraction effect is ideal,laying a foundation for the subsequent studies on total organic acids from Picris hieracioides L..关键词
毛连菜/总有机酸/响应面法/差示分光光度法/提取工艺Key words
Picris hieracioides L./Organic acid/Response surface method/Differential spectrophotometry/Extraction technology引用本文复制引用
王玫瑰,叶旭雯,叶方,赵文博,王雪芹,卢伟,黄良永..星点设计-响应面法优化毛连菜总有机酸提取工艺[J].湖北医药学院学报,2024,43(2):179-184,6.基金项目
十堰市科技局引导性科研项目(21Y03) （21Y03）
