橘红桔梗植物饮品对小鼠急性肺损伤和支气管哮喘的预防作用研究OA北大核心CSTPCD

AIM:This study aims to analyze the effects of Tangerine Platycodon Complex(TPC)on lipopoly-saccharide-induced acute lung injury and ovalbumin-induced bronchial asthma in mice,as well as its mechanism of ac-tion.METHODS:(1)Forty-eight male C57BL/6 mice were randomly divided into normal,acute lung injury,low,medi-um,and high dose(0.8,1.6 and 3.2 mL/d)of TPC groups,which were administered by gavage once a day for one week.Dexamethasone was used as a positive control.The acute lung injury mouse model was established through intratra-cheal administration of lipopolysaccharide.The pathological changes in lung tissue were observed using hematoxylin and eosin(HE)staining.The number of neutrophils in alveolar lavage fluid was determined using flow cytometry.ELISA was used to determine the concentrations of tumor necrosis factor α(TNF-α),interleukin 1β(IL-1β),IL-6 and IL-10,and to detect myeloperoxidase(MPO)activity in lung tissue homogenate and the protein content of I-κBα and NF-κB in lung tis-sue.(2)Forty-eight female C57BL/6 mice were randomly divided into the following groups:normal,bronchial asthma,low-,medium-,and high-dose groups(0.8,1.6 and 3.2 mL/d)of TPC,and the dexamethasone group.The mice were orally administered once a day for one week.A mouse model of bronchial asthma was established by intraperitoneal injec-tion of ovalbumin for sensitization and nasal stimulation.HE and toluidine blue staining were used to observe pathological changes in lung tissue,and the concentrations of IgE,IL-5,and IL-13 in mouse serum were determined using ELISA.RESULTS:Compared to the control group,mice in the acute lung injury group exhibited significant inflammatory cell in-filtration in the lung tissue.In bronchoalveolar lavage fluid(BALF),the percentage of neutrophils and MPO activity,and TNF-α,IL-1β,and IL-6 levels increased significantly(P<0.05),while IL-10 concentration decreased(P<0.05).West-ern blot revealed that the protein content of I-κBα decreased while NF-κB phosphorylation increased(P<0.05).Inflam-mation of lung tissue in the high-dose TPC and dexamethasone groups was significantly reduced.The percentage of neutro-phils,MPO activity in BALF,and the levels of TNF-α,IL-1β,and IL-6 significantly decreased(P<0.05),whereas IL-10 levels increased(P<0.05).The protein content of I-κBα increased in the lung tissue,whereas the levels of NF-κB phosphorylation were reduced(P<0.05).Compared to the normal group,mice in the bronchial asthma group exhibited significant inflammatory cell and mast cell infiltration.IgE and IL-13 levels in mouse serum increased(P<0.05).The pathological inflammatory cells and mast cells in the lung tissue of mice in the high-dose TPC and dexamethasone groups decreased compared to those in the bronchial asthma group,while mouse serum IgE levels decreased(P<0.05).More-over,IL-13 levels in dexamethasone-treated mice decreased(P<0.05).CONCLUSION:The acute lung injury induced by lipopolysaccharide in mice can be partially prevented by TPC.Additionally,it improved allergic reactions in mice with bronchial asthma by inhibiting the inflammatory response and reducing mast cell infiltration and IgE production.