中国动物检疫2024，Vol.41Issue(4)：58-63,90,7.DOI:10.3969/j.issn.1005-944X.2024.04.012
微滴式数字PCR技术在动物疫病检测中的应用研究进展
Research Progress in the Application of Droplet-based Digital PCR Assay to Animal Disease Detection
冯杰 1聂洪波 2曹乾大 3朱坤华 3敖元富 3黎春秀3
作者信息
- 1. 富顺县畜牧兽医技术推广中心,四川富顺 643200||富顺县动物疫病预防控制中心,四川富顺 643200
- 2. 富顺县农业综合管理站,四川富顺 643200
- 3. 富顺县动物疫病预防控制中心,四川富顺 643200
- 折叠
摘要
Abstract
Digital PCR(dPCR)is the third generation PCR developed recently,especially droplet-based digital PCR(ddPCR),with the advantages of high sensitivity,high tolerance,good accuracy,good reproducibility as well as absolute quantification for target nucleic acids in sample without relying on the standard curve,has been widely concerned by researchers.With a number of advantages,ddPCR has been rapidly developed in the field of animal disease pathogen detection,and successfully used to detect viruses,bacteria,parasites,Mycoplasma,Chlamydia and other pathogens,especially supporting the investigation into pathogens at low abundance,laboratory testing and typing identification.In the study,the general principles of ddPCR as well as its application and prospects in animal disease diagnosis were reviewed,with a view to providing a reference for application of the technology to animal disease diagnosis in veterinary laboratories.关键词
微滴式数字PCR/绝对定量/兽医实验室/动物疫病诊断Key words
ddPCR/absolute quantification/veterinary laboratory/animal disease diagnosis分类
农业科技引用本文复制引用
冯杰,聂洪波,曹乾大,朱坤华,敖元富,黎春秀..微滴式数字PCR技术在动物疫病检测中的应用研究进展[J].中国动物检疫,2024,41(4):58-63,90,7.
