应用低温等离子技术灭活体外结核分枝杆菌效果的初步研究OA北大核心CSTPCD

Objective:Preliminary investigation of the killing effect of low-temperature plasma technology on different Mycobacterium tuberculosis in vitro experiments,aiming to provide a new technical method for killing common Mycobacterium tuberculosis.Methods:Mycobacterium tuberculosis H37Rv strain,extensive drug-resistant Mycobacterium tuberculosis(XDR-MTB)and multidrug-resistant Mycobacterium tuberculosis(MDR-MTB)mycobacterial fluids were inoculated on the culture medium,configured as a 0.5 mycobacterial suspensions within 3 weeks of incubation,and then multiply diluted to the test mycobacterial suspensions,and 2.0 ml of mycobacterial suspension samples were taken into sterile test tubes,the suspensions were ablated for 0,3,6,9,12 and 15 s by PLACOAG mode of low-temperature plasma surgery system,and then cultured in incubators.After 21 days,the number of the mean survival count,log10 reduction,and killing efficiency of each group at each treatment time point were calculated.Results:(1)The log10 reduction of H37Rv(F=20.313,P=0.003),XDR-MTB(F=13.956,P=0.006),and MDR-MTB(F=20.355,P=0.006)were statistically different at different treatment times.There was a statistically significant difference in the log10 reduction of three bacterial groups at 9 seconds(F=61.603,P=0.004),with XDR-MTB was the lowest(0.50±0.00),followed by H37Rv(1.14±0.12),and MDR-MTB was the highest(1.23±0.00),and the log10 reduction of the three groups reached the maximum value at 15 seconds,which were MDR-MTB(1.71±0.00),H37Rv(1.83±0.00),XDR-MTB(1.35±0.21).(2)The killing efficiency of H37Rv(F=10.458,P=0.012),XDR-MTB(F=10.945,P=0.011),and MDR-MTB(F=9.424,P=0.015)were statistically different at different treatment times.There was a statistically significant difference in the killing efficiency of three bacterial groups at 9 seconds(F=287.890,P＜0.001),with XDR-MTB was the lowest((68.25±0.00)％),followed by H37Rv((92.59±2.10)％),and MDR-MTB was the highest((94.12±0.00)％),and the killing efficiency of the three groups reached the maximum value at 15 seconds,which were MDR-MTB(100.00±0.00)％,H37Rv(96.26±1.05)％,XDR-MTB(99.35±5.16)％.Conclusion:In this study,in vitro experiments confirmed that low-temperature plasma technique has a rapid and significant killing effect on three Mycobacterium tuberculosis species,and that the killing effect varies and requires individualized strategy.