中国农业科学2024，Vol.57Issue(8)：1575-1591,17.DOI:10.3864/j.issn.0578-1752.2024.08.012
转录组和蛋白质组关联分析解析巴西蕉幼苗响应低温的分子机制
Transcriptome and Proteome Association Analysis to Revealthe Molecular Mechanism of Baxi Banana Seedlings in Response to Low Temperature
摘要
Abstract
[Objective]Low temperature is a significant natural disaster that affects banana production.In this study,based on transcriptome and proteome association analysis,the regulatory network of genes,proteins,signals and metabolic pathways involved in banana cold resistance was investigated.The aim was to explore the molecular mechanism of banana cold resistance.[Methods]'Baxi'banana(Musa nana Lour)was treated at 7 ℃ for 1 and 3 d,and a control group was treated at 28 ℃.Based on the proteome data obtained in the previous study,the transcriptome sequencing technology was used to detect changes in the gene regulatory network of banana under cold stress.Simultaneously,the correlation analysis was conducted with proteomics to analyze the molecular mechanism of banana response to cold stress.[Result]Transcriptome analysis revealed that 11 370,15 460 and 9 619 differentially expressed genes were identified in the three comparison groups of Coldl vs CK,Cold3 vs CK and Coldl vs Cold3,respectively.KEGG enrichment analysis of these genes revealed that the differentially expressed genes were enriched in several key signaling metabolic pathways,such as photosynthesis signal,glutathione metabolism,α-linolenic acid metabolic pathway and phenylpropanoid biosynthesis under low temperature stress.Moreover,there were significant differences in the enrichment degree of glutathione metabolic pathway between Coldl d vs CK and Cold3 d vs CK.Real-time quantitative PCR analysis(qRT-PCR)was performed on several differentially expressed genes.Among them,the expression levels of key low-temperature regulatory genes,such as DREB,MAPK and MYB,were significantly increased after the low-temperature treatment.The expression trend of the selected 20 genes was essentially consistent with that of RNA-seq,confirming the accuracy of RNA-seq.The results of the transcriptome and proteome association analysis showed a positive correlation between the transcriptome and proteomics.A total of 6 211 proteins corresponding to transcripts were identified.Among these,105 transcripts and their proteins were up-regulated,while 218 transcripts and their proteins were down-regulated.GO enrichment analysis showed that the differentially expressed genes and proteins were enriched in functions,such as photoresponse,chloroplast and oxidoreductase activity.Furthermore,the correlation analysis of differentially expressed genes and protein KEGG pathway revealed that the low temperature treatment suppressed the expression of genes and proteins related to phenylpropanoid biosynthesis and photosynthesis signaling pathway,while promoting the expression of proteins associated with α-linolenic acid metabolism and the glutathione pathway.[Conclusion]The transcriptome and proteomics were used to map the regulatory network of banana cold resistance at the gene and protein levels.It was found that the signal pathway of banana response to low temperature mainly involved photosynthesis signal,glutathione metabolism,α-linolenic acid metabolism and phenylpropanol biosynthesis.关键词
香蕉/低温胁迫/转录组/蛋白质组/关联分析Key words
Musa nana Lour/low temperature stress/transcriptome/proteome/comparative analysis引用本文复制引用
林蔚,吴水金,李跃森..转录组和蛋白质组关联分析解析巴西蕉幼苗响应低温的分子机制[J].中国农业科学,2024,57(8):1575-1591,17.基金项目
福建省公益类科研院所专项(2022R1030006,2021R1030006)、福建省自然科学基金(2023J01374) （2022R1030006,2021R1030006）
