济南市两株输入性登革热3型病例病毒基因组全序列测定及分析OA北大核心CSTPCD
Determination and analysis of complete genomic sequences of two Dengue 3 virus strains from imported cases in Jinan
目的 对济南市两株输入性登革热病例进行血清学和基因检测,获取全基因数据并进行分子特征分析.方法 采集济南市2例登革热疑似阳性病例的全血样本,通过Real-time PCR对病毒进行分型检测.离心分离血清,用于胶体金法检测登革病毒NS1抗原、IgM抗体和IgG抗体;同时扩增病毒全基因序列,将序列与国内外分离株序列进行比对,Mega 7.0软件构建进化树,RDP4软件进行基因重组分析.结果 2例病例核酸分型检测结果为DENV3型,胶体金检测结果显示N…查看全部>>
This study was aimed at identifying the serotypes and genotypes of two dengue fever virus strains isolated from two imported cases of dengue fever in Jinan city;obtaining their whole genome sequences;and analyzing their molecular evolu-tionary characteristics.Whole blood samples from two suspected positive cases of dengue fever in Jinan city were collected,and the subtypes were identified by real-time PCR.Serum samples were collected to detect dengue virus I…查看全部>>
韩莹;焦海涛;刘子薇;武晶晶;刘岚铮
济南市疾病预防控制中心,济南 250021济南市疾病预防控制中心,济南 250021济南市疾病预防控制中心,济南 250021济南市疾病预防控制中心,济南 250021济南市疾病预防控制中心,济南 250021
基础医学
登革病毒全基因序列系统进化分析
dengue fever viruswhole genome sequencephylogenetic analysis
《中国人兽共患病学报》 2024 (3)
253-256,4
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