中国蔬菜Issue(4)：121-128,8.DOI:10.19928/j.cnki.1000-6346.2024.5005
哈茨木霉β-葡聚糖酶诱导、纯化及对黄瓜幼苗的促生防病作用
Induction and Purification of β-Glucanase from Trichoderma harzianum and Its Growth Promoting and Disease Prevention Effects on Cucumber Seedlings
摘要
Abstract
Under laboratory condition,this experiment took β-glucan,wheat bran and cell wall of Fusarium oxysporum as the only carbon source to induce Trichoderma harzianum Th-30 fermentation and β-glucanase production,and isolate and purify β-glucanase by ammonium sulfate salting out and agarose gel column chromatography.Taking'Jinyan 4'cucumber as test material,this experiment also explored the effects of β-glucanase on physiological and morphological indexes of resistance,and prevention and control function of cucumber seedling on common soil-borne diseases of cucumber.The results showed that the activities of β-glucanase from Trichoderma Th-30 fermentation induced under different conditions were significantly different.The enzyme activity of crude enzyme liquid induced by β-glucan was the highest,reaching 75.63 U·mL-1 at 72 h of fermentation.The specific activity of purified β-glucanase was 783.56 U·mg-1,being 45.32 times of the crude enzyme.The treatments of 5-fold solution and 10-fold solution of β-glucanase could increase the root activity and free proline content,PAL,POD,and PPO activities of cucumber seedling,as well as the root shoot ratio and healthy seedling strength index,and the control effects of 10-fold solution of β-glucanase on cucumber Rhizoctonia root rot,cucumber Fusarium wilt,cucumber blight,cucumber damping-off,cucumber stem rot were 50.36%-80.63%.关键词
哈茨木霉/β-葡聚糖酶/分离纯化/黄瓜幼苗/生防作用Key words
Trichoderma harzianum/β-glucanase/separation and purification/cucumber seedling/biocontrol function引用本文复制引用
杨春林,李洪浩,胡强,席亚东..哈茨木霉β-葡聚糖酶诱导、纯化及对黄瓜幼苗的促生防病作用[J].中国蔬菜,2024,(4):121-128,8.基金项目
国家现代农业产业体系四川创新团队(川农函[2019]472号),四川省科技计划项目(2021YFYZ0021),四川蔬菜花卉种植创新与产业提升关键技术研发专项(2016CYTS-018) （川农函[2019]472号）
