中国输血杂志2024，Vol.37Issue(4)：399-404,6.DOI:10.13303/j.cjbt.issn.1004-549x.2024.04.005
自制输血相容性检测低值阳性质控品的方法学建立
Establishment of internal quality control methodology for blood transfusion compatibility testing
摘要
Abstract
Objective To monitor the effectiveness and accuracy of the blood transfusion compatibility test system by self-made weakly positive internal quality control products.Methods Red blood cells from DAT(-)healthy subjects were selected,and B/RhD(-)E(-)red blood cells were selected as tube 1.A/RhD(+)E(+)was selected as tube 2 to prepare blood group quality control products according to the principle of blood group antigen compatibility,and red blood cell pres-ervation solution and corresponding ABO blood group reagent antibody were added to make the agglutination intensity of mi-crocolumn gel method in reverse blood typing reach a low positive value(1+).Tube 3 and tube 4 were prepared with five different preservation media:plasma,serum,antibody diluent,mixture of equal plasma and antibody diluent,and mixture of equal serum and antibody diluent,respectively.IgM anti-E antibody was added to tube 3,and IgG anti-D antibody was add-ed to tube 4,so that the agglutination intensity of microcolumn gel method reached a low positive value(1+).Results Comparison between the 5 different preservation media showed that the preservation medium of antibody diluent was the most stable for weakly positive antibody(F=11.35,P<0.05),Agglutination intensity 1+is assigned 5 points by AABB Technical Manual,and its score was 5.25±1.75 points.Conclusion The use of self-made weakly positive quality control products can improve the effectiveness,accuracy and sensitivity of the monitoring system,thus achieving internal quality control and en-suring the safety of clinical blood use.关键词
室内质控品/输血相容性检测/低值阳性/剂量效应/Rh表型频率Key words
quality control products/transfusion compatibility testing/weakly positive/dose effect/Rh phenotype fre-quency分类
临床医学引用本文复制引用
李璐,孙晓琳,魏俊杰,刘瑞琪,吴伟鑫,刘海云,张印则..自制输血相容性检测低值阳性质控品的方法学建立[J].中国输血杂志,2024,37(4):399-404,6.基金项目
深圳市南山区卫生科技项目(NS2021164) （NS2021164）
