中国输血杂志2024，Vol.37Issue(4)：412-416,5.DOI:10.13303/j.cjbt.issn.1004-549x.2024.04.007
HBsAg ELISA+/HBV DNA NAT-献血者血清学与分子生物学特征分析
Serological characteristics of HBsAg positive/HBV DNA non-reactive blood donors
摘要
Abstract
Objective To explore the distribution of serological markers related to samples whose serological test results were inconsistent with HBV DNA test results among voluntary blood donors in Xi'an.Methods A total of 71 HBsAg ELISA positive and NAT non-reactive(ELISA+/NAT-)blood samples were collected from Shaanxi Blood Center from November 1,2022 to April 30,2023.The serological markers of hepatitis B were detected by electrochemiluminescence method,and the HBV S region and C region gene fragments were amplified by nested-PCR.Results The positive rate of nested-PCR in double ELISA+/NAT-group(n=30)was statistically higher than that of ELISA+/NAT-group(n=41)(60%vs 24.4%,P<0.05).Donors in double ELISA+/NAT-group were all first-time blood donors,with the positive rate of anti-HBc in serum of100%,and the serological pattern was mainly positive for items 1,4 and 5 items(80%).Among the ELISA+/NAT-group,31.7%were repeat blood donors,with the positive rate of anti-HBc in serum of only 19.51%,and the serological patterns were mainly single anti-HBs positive(43.90%)and all negative(36.58%).Conclusion There are false positives in the test results of ELISA+/NAT-group,which leads to unnecessary blood discarding.Meanwhile,the samples with nega-tive NAT may have low levels of HBV DNA,which may lead to missed detection.It is suggested that multiple systems and methods should be applied to trace the blood donors who are HBsAg positive and NAT non-reactive,so as to improve the ac-curacy of HBV screening of blood donors and reduce blood waste.关键词
乙型肝炎表面抗原/无偿献血者/巢式PCR/HBV DNAKey words
HBsAg/voluntary blood donors/nested-PCR/HBV DNA分类
医药卫生引用本文复制引用
景媛媛,范云,郭燕,张文娟,段勇,冯娜..HBsAg ELISA+/HBV DNA NAT-献血者血清学与分子生物学特征分析[J].中国输血杂志,2024,37(4):412-416,5.基金项目
西安市卫健委一般研究项目(2020yb48) （2020yb48）
