中国输血杂志2024，Vol.37Issue(4)：417-424,8.DOI:10.13303/j.cjbt.issn.1004-549x.2024.04.008
标本溶血对血站血液筛查实验室ELISA检测结果的影响
Influence of sample hemolysis on ELISA test results in blood screening laboratory
秦倩倩 1葛红卫 1张婧 1赵子复 1冷婵 1王瑞1
作者信息
- 1. 北京市红十字血液中心,北京 100088
- 折叠
摘要
Abstract
Objective To analyze the effect of sample hemolysis on ELISA test results in blood screening laboratory,so as to determine the acceptable tolerance of hemolysis specific to laboratory test items and detection system,and provide ref-erence for the formulation of tolerance standard of sample hemolysis.Methods Negative and weakly positive(S/CO was a-bout 2)samples with different hemolysis degrees were tested by several commonly used domestic reagents for HBsAg,HIV Ag/Ab,anti-HCV and anti-TP,respectively.The effects of various degrees of hemolysis on the test results of negative and weakly positive samples for each item were analyzed.Results 1)Hemolysis had no effect on the test results(reactive/non-reactive)of negative and weakly positive samples for HBsAg,anti-HCV and anti-TP ELISA items;2)Hemolysis affected the test results(reactive/non-reactive)of negative and weakly positive samples for HIV Ag/Ab ELISA item.A tolerance of Hb 2 g/L was taken as the acceptable hemolysis degree for HIV Ag/Ab ELISA item.Conclusion In this study,the accept-able tolerance of hemolytic samples for corresponding test items and detection system in our laboratory were determined.The influence of hemolysis on ELISA test result is related to the reagent,equipment,environment and other factors,therefore the acceptable tolerance of hemolysis should be determined scientifically and reasonably based on the specific evaluation of each laboratory.关键词
溶血/血站实验室/ELISAKey words
hemolysis/blood screening laboratory/ELISA分类
医药卫生引用本文复制引用
秦倩倩,葛红卫,张婧,赵子复,冷婵,王瑞..标本溶血对血站血液筛查实验室ELISA检测结果的影响[J].中国输血杂志,2024,37(4):417-424,8.
