中国实用口腔科杂志2024，Vol.17Issue(2)：199-205,7.DOI:10.19538/j.kq.2024.02.012
伴2型糖尿病牙周炎患者唾液菌群特征分析
Characteristics analysis of salivary microbiota in patients with periodontitis and type 2 diabetes mellitus
摘要
Abstract
Objective To analyze the salivary microbiota composition and diversity changes in patients with type 2 dia-betes mellitus(T2DM)and periodontitis.Methods Whole saliva samples in a non-stimulated state were collected from 18 patients with periodontitis(P group)and 19 patients with periodontitis and T2DM(DP group)who visited the Depart-ment of Periodontology at the Nanjing Stomatology Hospital of Nanjing University from March to December 2022.The 16S rRNA high-throughput sequencing was performed.The diversity and compositional differences between the two groups were compared using α-diversity analysis,β-diversity analysis,and linear discriminant analysis effect size(LEfSe).Additionally,the differences in metabolic pathway abundance and the relationship between bacterial abun-dance and fasting blood glucose(FBG)concentration were analyzed.Results Faith's PD index and Observed species in-dex in the DP group were significantly higher than those in the P group,with statistically significant differences(Z values were-3.130 and-2.218 respectively,both P＜0.05).However,comparisons of other α-diversity indices between the two groups showed no statistical difference(all P＞0.05).β-diversity analysis revealed distinct clustering of salivary mi-crobiota in both groups.LEfSe analysis showed that Actinobacteria were significantly dominant in the DP group,while Fusobacteria and Synergistetesr were significantly dominant in the P group(all P＜0.05).Metabolic pathway analysis demonstrated sta-tistically significant differences in pathway abun-dance related to glucose oxidation,polymyxin resistance,amino acid metabolism,and cholinesterase metabolism(all P＜0.05).Abundances of Fusobacteria,Actinobacteria,and Synergistetesr were significantly correlated with FBG(all P＜0.05).Conclusion Compared to periodontitis patients without T2DM,those with both conditions exhibit characteristic alterations in the composition of salivary microbiota and metabolic pathways of the microbiome.These changes might be associated with the progression of T2DM.关键词
2型糖尿病/慢性牙周炎/唾液菌群/16S rRNAKey words
type 2 diabetes mellitus/chronic periodontitis/salivary microbiota/16S rRNA分类
医药卫生引用本文复制引用
孔境崧,王敏,金伟秋,闫福华,李艳芬..伴2型糖尿病牙周炎患者唾液菌群特征分析[J].中国实用口腔科杂志,2024,17(2):199-205,7.基金项目
南京大学医学院附属口腔医院2015学科带头人后备人才资助项目(0223A202) （0223A202）
江苏省医学重点学科建设单位项目(JSDW202246) （JSDW202246）
