高糖新品种云蔗1640花叶病原的检测与脱毒种苗生产OA

[Objective]Sugarcane Mosaic Disease is a serious disease leads to the yield decline of sugarcane varieties,and the disease resistance of sugarcane seedlings can be effectively improved by applying healthy sugarcane seedlings.'Yunzhe 1640'is a new sugarcane variety with high yield and high sugar developed by the Sugarcane Research Institute of Yunnan Academy of Agricultural Sciences,suitable for popularization and planting in tropical areas in China.[Methods]In this study,field sampling and RT-PCR detection were used to complete the investigation of Mosaic virus disease of the sugarcane variety'Yunzhe 1640'.The virus-free treatment and rapid propagation of'Yunzhe 1640'healthy seedlings were also carried out.[Results]It was confirmed that'Yunzhe 1640'has the Sugarcane mosaic virus(SCMV).The main pathogen was sugarcane steak mosaic virus(SCSMV),followed by SCMV,without sorghum mosaic virus(SrMV).The healthy seedling of'Yunzhe 1640'was obtained by hot water treatment with detoxification using axillary meristem culturing.The results showed that the detoxification efficiency of the SCSMV and SCMV detected by RT-PCR was 10.12%and 90.62%,respectively.Virus-free seedlings without pathogen were used as original seedlings for tissue culture and rapid propagation of healthy seedlings.[Conclusion]Healthy sugarcane seedling propagation is an effective breeding technique for elite sugarcane varieties.It can accelerate the promotion and application of elite new sugarcane varieties.It can also provide new elite germplasms for the development of the cane sugar industry in China and is a new source of purification and rejuvenation of old cane varieties in sugarcane-growing areas in China.