中国糖料2024，Vol.46Issue(2)：9-16,8.DOI:10.13570/j.cnki.scc.2024.02.002
甜菜BvPCNA基因RNAi载体构建
Construction of RNAi Vector of BvPCNA Gene in Sugar Beet
摘要
Abstract
[Objective]Proliferating cell nuclear antigen(PCNA)is widely existed in plants,but its function in plants has been rarely studied.This study could lay the foundation for the functional analysis of BvPCNA-like genes.[Methods]A gene site in the sugar beet genome had been isolated in the previous study,and named as BvPCNA-like gene.In this study,the cloning vector of BvPCNA-like gene was used as the initial material to select the appropriate target sequence.The plant expression vector pFGC5941 was used as the basic vector to make sure the upstream promoter of target sequence was 35 S,and the plant screening marker is the herbicide-resistant gene BlpR.Target-specific primers were designed and the BvPCNA-RNAi vector was constructed by PCR-enzyme digestion-linkage reactions.[Results]The PCR identification showed that the expected vector had been obtained,which was composed of forward target BvPCNA-F,intermediate segment,and reverse target BvPCNA-R,with a target sequence length of 320 bp.[Conclusion]The vector obtained in this study can be used for genetic transformation of dicotyledonous plants,and as a vector material for theoretical research or application research of BvPCNA-like gene.关键词
甜菜/BvPCNA-like/RNAi/载体构建Key words
sugar beet/BvPCNA-like/RNAi/vector construction分类
农业科技引用本文复制引用
王希,张西宁,陶艳昵,张琦,李志烨,赵春雷..甜菜BvPCNA基因RNAi载体构建[J].中国糖料,2024,46(2):9-16,8.基金项目
博士后研究人员落户黑龙江科研启动资助金"甜菜分子标记开发与重要性状相关标记的筛选"(LBH-Q18108) （LBH-Q18108）
2017年度黑龙江省省属高等学校基本科研业务费科技创新类重点项目"甜菜分子标记位点库的深度挖掘与候选SSR标记开发"(KJCXZD201713) （KJCXZD201713）
财政部和农业农村部国家现代农业产业技术体系(糖料)建设项目"甜菜种质资源收集与评价"(CARS-170102)资助. （糖料）
