中国糖料2024,Vol.46Issue(2):17-24,8.DOI:10.13570/j.cnki.scc.2024.02.003
24份农艺性状优异割手密黑穗病抗性鉴定及Bru1基因分子检测
Identification of Resistance to Smut and Molecular Detection of Bru1 Gene in 24 Elite Saccharum spontaneum L.
摘要
Abstract
[Objective]The aim of this study was to screen Saccharum spontaneum L.(S.spontaneum L.)germplasm resistant to smut and brown rust,so as to provide theoretical references for the research on disease-resistant parents for breeding for resistance to smut and brown rust in sugarcane.[Methods]The identification resistance to smut for 24 elite S.spontaneum L.was conducted by the method of dipping inoculation,and the resistance to brown rust was conducted by testing two molecular markers Bru1 closely linked to brown rust gene.[Results]The results showed that the test materials showed strong resistance to smut,16 performed high resistance at level 1,5 materials performed resistance at level 2,and 3 materials performed resistance at level 3;The molecular detection indicated that 7 materials contained the Bru1 gene,accounting for 29.17%of all the tested materials.[Conclusion]The germplasms selected in this study with high resistance to smut and containing Bru1 gene are good sources for developing sugarcane varieties(parents)with resistance to smut and brown rust disease.关键词
割手密/黑穗病/褐锈病/抗性鉴定Key words
Saccharum spontaneum L./smut/brown rust/resistance identification分类
农业科技引用本文复制引用
黄玉新,高轶静,张革民,张保青,黄冬梅,陆衫羽,颜梅新,高丽花,周珊,杨翠芳,段维兴,张小秋..24份农艺性状优异割手密黑穗病抗性鉴定及Bru1基因分子检测[J].中国糖料,2024,46(2):17-24,8.基金项目
广西科技重大专项(桂科AA22117002-1) (桂科AA22117002-1)
广西自然科学基金项目(2023GXNSFAA026354,2023GXNSFAA026088) (2023GXNSFAA026354,2023GXNSFAA026088)
中国科学院华南植物园重点支持项目(E36101) (E36101)
广西农业科学院基本科研业务专项项目(桂农科2021YT004、2023YM53、2024YP028) (桂农科2021YT004、2023YM53、2024YP028)
广西重点实验室建设项目系统性研究课题(21-238-16-A-01-02)资助. (21-238-16-A-01-02)