24份农艺性状优异割手密黑穗病抗性鉴定及Bru1基因分子检测OA

[Objective]The aim of this study was to screen Saccharum spontaneum L.(S.spontaneum L.)germplasm resistant to smut and brown rust,so as to provide theoretical references for the research on disease-resistant parents for breeding for resistance to smut and brown rust in sugarcane.[Methods]The identification resistance to smut for 24 elite S.spontaneum L.was conducted by the method of dipping inoculation,and the resistance to brown rust was conducted by testing two molecular markers Bru1 closely linked to brown rust gene.[Results]The results showed that the test materials showed strong resistance to smut,16 performed high resistance at level 1,5 materials performed resistance at level 2,and 3 materials performed resistance at level 3;The molecular detection indicated that 7 materials contained the Bru1 gene,accounting for 29.17%of all the tested materials.[Conclusion]The germplasms selected in this study with high resistance to smut and containing Bru1 gene are good sources for developing sugarcane varieties(parents)with resistance to smut and brown rust disease.