circRNA CCND1对人肝癌HepG2细胞增殖、凋亡、侵袭和迁移的影响OACSTPCD
Effects of circRNA CCND1 on proliferation,apoptosis,invasion and migration of human hepatocellular carcinoma HepG2 cells
目的 探讨circRNA CCND1对人肝癌HepG2细胞增殖、凋亡、侵袭和迁移的影响.方法 采用荧光原位杂交(FISH)实验及实时荧光定量聚合酶链反应(qRT-PCR)检测circRNA CCND1在HepG2细胞及L02细胞中的表达情况;进一步干扰及过表达circRNA CCND1后,通过qRT-PCR检测circRNA CCND1在HepG2细胞中的表达情况,CCK-8法和EDU法检测HepG2细胞增殖情况,流式细胞术检测细胞凋亡、细胞周期…查看全部>>
Objective To investigate the effects of circRNA CCND1 on the proliferation,apoptosis,invasion and migration of human hepatocellular carcinoma HepG2 cells.Methods The expression of circRNA CCND1 in HepG2 and LO2 cells was detected by fluorescence in situ hybridization(FISH)and quantitative real-time polymerase chain reaction(qRT-PCR).After further interference of the expression of circRNA CCND1 or overexpression of circRNA CCND1,the level of circRNA CCND1 in …查看全部>>
董辉;顾铃毓;俞梦楚;张旭
宁夏医科大学总医院医学科学研究院,宁夏 银川 750000宁夏医科大学总医院心脑血管病医院 医学检验科,宁夏 银川 750000宁夏医科大学总医院医学科学研究院,宁夏 银川 750000宁夏医科大学总医院医学科学研究院,宁夏 银川 750000
临床医学
肝肿瘤circRNA CCND1HepG2细胞生理学现象
hepatocellular carcinomacircRNA CCND1HepG2cell physiological phenomenon
《中国现代医学杂志》 2024 (8)
1-8,8
国家自然科学基金青年项目(No:82002955)
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