中国现代医学杂志2024，Vol.34Issue(8)：21-27,7.DOI:10.3969/j.issn.1005-8982.2024.08.004
针刺对乳腺癌化疗后癌因性疲乏模型小鼠肠-脑轴相关因子的影响
Effect of acupuncture on gut-brain axis-associated molecular profiles in mouse models of cancer-related fatigue after chemotherapy for breast cancer
摘要
Abstract
Objective To observe the effect of acupuncture on gut-brain axis-associated molecular profiles in mouse models of cancer-related fatigue(CRF)after chemotherapy for breast cancer,and to explore the possible mechanism underlying the improvement of CRF in breast cancer mediated by acupuncture.Methods BALB/c mice were randomly divided into blank group,model group,acupuncture group and sham acupuncture group.The tumor-bearing mice subjected to chemotherapy were used to establish models of CRF after chemotherapy for breast cancer.Acupoints including"Zusanli","Sanyinjiao","Baihui","Guanyuan",and"Qihai"were selected for acupuncture,and the mice underwent 30-minute treatment once a day for 14 days.The physical strength and fatigue of mice were evaluated through general conditions,forced swimming tests,and open field tests.Western blotting was performed to detect the protein expressions of inflammatory factors IL-1β,IL-6,and TNF-α in hypothalamus and colon tissues.ELISA was used to determine the serum levels of CORT and ACTH associated with the hypothalamic-pituitary-adrenal(HPA)axis.Results The immobility time during the forced swimming tests in the model group was longer than that in the blank group(P<0.05).Compared with the acupuncture group,the immobility time during the forced swimming tests before and after the intervention was longer in the model group(P<0.05).Besides,the immobility time during the forced swimming tests in the sham operation group was longer than that in the acupuncture group(P<0.05).The scores of horizontal and vertical movements in the model group were lower than those in the blank group(P<0.05).The scores of horizontal and vertical movements in the acupuncture group were higher than those in the model group and the sham acupuncture group(P<0.05).The relative protein expressions of IL-1β,IL-6,and TNF-α in the hypothalamus of mice in the model group and the sham acupuncture group were higher than those in the blank group(P<0.05),while those in the acupuncture group were lower relative to those in the model group and the sham acupuncture group(P<0.05).The relative protein expressions of IL-1β,IL-6,and TNF-α in the colon tissues of mice in the acupuncture group,model group and the sham acupuncture group were all higher than those in the blank group(P<0.05),those in the acupuncture group were lower than those in the model group(P<0.05),and those in the acupuncture group were lower than those in the sham acupuncture group(P<0.05).The protein level of ACTH in the model group and the sham acupuncture group was higher than that in the blank group(P<0.05),whereas the protein level of CORT in the model group and the sham acupuncture group was lower than that in the blank group(P<0.05).The protein level of ACTH in the acupuncture group was lower than that in the model group(P<0.05),whereas the protein level of CORT in the acupuncture group was higher than that in the model group(P<0.05).The protein level of ACTH in the acupuncture group was lower than that in the sham acupuncture group(P<0.05),whereas the protein level of CORT in the acupuncture group was higher than that in the sham acupuncture group(P<0.05).Conclusion Acupuncture could mitigate the fatigue in mouse models of CRF after chemotherapy for breast cancer,possibly by reducing the expressions of proinflammatory factors in the central nervous system and the colon and modulating the dysfunction of the HPA axis,which may also be part of the mechanisms underlying the roles of acupuncture in treating CRF.关键词
乳腺癌/癌因性疲乏/下丘脑/结肠/炎症因子/HPA轴Key words
breast cancer/cancer-related fatigue/hypothalamus/colon/inflammatory factors/HPA axis分类
医药卫生引用本文复制引用
刘瑞东,王泽鹏,冯晓东,程旭锋,房璐,阮晓迪,聂晨晨,苏凯奇,吕转..针刺对乳腺癌化疗后癌因性疲乏模型小鼠肠-脑轴相关因子的影响[J].中国现代医学杂志,2024,34(8):21-27,7.基金项目
河南省中医药科学研究专项课题项目(No:2022ZY1019) （No:2022ZY1019）
