中国药房2024，Vol.35Issue(8)：918-924,7.DOI:10.6039/j.issn.1001-0408.2024.08.04
桃叶珊瑚苷对乳腺癌细胞恶性生物学行为的影响及机制
Effects and mechanism of aucubin on malignant biological behavior of breast cancer cells
摘要
Abstract
OBJECTIVE To investigate the effects of aucubin(Auc)on the malignant biological behavior of breast cancer cells by regulating cyclin-dependent kinase 1(CDK1)/cyclin B1(CCNB1)/Polo-like kinase 1(PLK1)signaling pathway.METHODS Human breast cancer cells MCF-7 were divided into control group,Auc low-,medium-and high-concentration groups(AUC-L,AUC-M,AUC-H groups,20,40 and 80 μmol/L Auc),Auc-H+pcDNA-NC group(80 μmol/L Auc+transfected pcDNA-NC plasmid),and Auc-H+pcDNA-CDK1 group(80 μmol/L Auc+transfected pcDNA-CDK1 plasmid).Cell proliferation,clonal formation,invasion and migration abilities,apoptosis and cycle distribution,and the expressions of related proteins of apoptosis,epithelial-mesenchymal transformation(EMT)and CDK1/CCNB1/PLK1 signaling pathway were detected in each group.The transplanted tumor model of BALB/c nude mice was established by subcutaneous inoculation of MCF-7 cell suspension,and the mice were divided into control group and Auc group(12 mice in each group).The tumor volume,mass and the expressions of related proteins of CDK1/CCNB1/PLK1 signaling pathway in tumor tissues were detected.RESULTS Compared with control group,the number of clonal formation,proliferation rate,cell invasion number,scar healing rate,G1/G0 phase and S phase cell proportions,and the expressions of B cell lymphoma-2(Bcl-2),N-cadherin,fibronectin,CDK1,CCNB1 and PLK1 were decreased significantly(P＜0.05).The apoptotic rate,G2/M phase cell proportion and the expressions of Bcl-2 associated X protein and E-cadherin were significantly increased,in a dose-dependent manner(P＜0.05).Compared with the Auc-H+pcDNA-NC group,there was no statistical significance in the above indexes in the Auc-H group(P＞0.05),while the above indexes in the Auc-H+ pcDNA-CDK1 group were significantly reversed(P＜0.05).Compared with the control group,the tumor volume and mass,and the expressions of CDK1,CCNB1 and PLK1 in tumor tissue of Auc group were significantly decreased(P＜0.05).CONCLUSIONS Auc can inhibit the proliferation,invasion and migration of breast cancer cells,induce cell cycle arrest,and inhibit the progression of EMT,which may be related to inhibiting the activation of the CDK1/CCNB1/PLK1 signaling pathway.关键词
桃叶珊瑚苷/乳腺癌/恶性生物学行为/CDK1/CCNB1/PLK1信号通路Key words
aucubin/breast cancer/malignant biological behavior/CDK1/CCNB1/PLK1 signaling pathway分类
医药卫生引用本文复制引用
司运辉,蒋凯,钱立全,陈永顺,别会杰..桃叶珊瑚苷对乳腺癌细胞恶性生物学行为的影响及机制[J].中国药房,2024,35(8):918-924,7.基金项目
国家卫生健康委医药卫生科技发展研究中心课题(No.W2022ZT726) （No.W2022ZT726）
河南省高等学校重点科研项目(No.23B310015) （No.23B310015）
