中国医科大学学报2024，Vol.53Issue(4)：302-308,7.DOI:10.12007/j.issn.0258-4646.2024.04.003
BTG4在胃癌中的表达及临床意义
Expression and clinical significance of BTG4 in gastric cancer
摘要
Abstract
Objective To investigate the expression and clinical significance of BTG4 in the occurrence and progression of gastric cancer.Methods Oncomine,The Cancer Genome Atlas Program(TCGA),Xiantao database,and Kaplan-Meier platforms were used to analyze the expression of BTG4mRNA in gastric cancer and its relationship with various clinical indicators and patient prognoses.Paraffin samples from gastric adenocarcinoma tissues(n= 457)and normal mucosal tissues(n= 243,≥4 cm from adenocarcinoma tissues)were obtained from the First Affiliated Hospital of Jinzhou Medical University from January 2012 to January 2015.Real-time polymerase chain reaction(PCR)and Western blotting were performed to measure BTG4 expression,and the relationship between BTG4mRNA expression and various clinical indicators and patient prognoses was analyzed.Gastric adenocarcinoma and para-carcinoma tissues were used for micro assays,and BTG4 protein expression in each micro assay was analyzed using immunohistochemistry.In addition,the relationship between BTG4 protein expression and the occurrence,clinicopathological features,and prognoses of patients with gastric adenocarcinoma was analyzed.Results Compared with normal mucosal tissues,the expression of BTG4mRNA and protein in gastric cancer tissues was down-regulated(all P<0.05).The expression of BTG4mRNA was negatively correlated with overall survival and progression-free sur-vival(P<0.05).Young age,distant metastasis,and high expression of BTG4 were independent risk factors for poor prognosis in patients with gastric cancer(P<0.05).Immunohistochemical results showed that BTG4 expression was higher in gastric adenocarcinoma(59.5%,272/457)and intestinal metaplasia(20.6%,37/143)than in gastritis(13/100,13%;P<0.05).BTG4 expression in gastric papillary ade-nocarcinoma was higher than that in mucinous adenocarcinoma(P<0.05),and BTG4 expression in highly differentiated adenocarcinoma was higher than that in moderately differentiated adenocarcinoma(P<0.05).Conclusion BTG4 expression in gastric cancer tissues was negatively correlated with the overall survival of patients with gastric cancer.Positive expression of BTG4 is closely related to the occur-rence and development of gastric cancer.Therefore,BTG4 is a promising predictor of the prognosis of gastric cancer and a potential target for gene therapy in patients with gastric cancer.关键词
BTG4/胃癌/预后/生物信息学Key words
gastric cancer/BTG4/prognosis/bioinformatics分类
医药卫生引用本文复制引用
张聪宇,张文陆,郑华川..BTG4在胃癌中的表达及临床意义[J].中国医科大学学报,2024,53(4):302-308,7.基金项目
国家自然科学基金(81672700) （81672700）
