中国医科大学学报2024，Vol.53Issue(4)：342-348,354,8.DOI:10.12007/j.issn.0258-4646.2024.04.009
GDF11调控NFκ-B通路影响巨噬细胞M1极化介导的炎症反应
GDF11 affects M1 polarization mediated inflammatory response in macrophages by regulating the NF-κB pathway
摘要
Abstract
Objective To investigate the effect of GDF11 on the macrophage M1 polarization mediated inflammatory response and its mechanism.Methods RAW264.7 macrophages were cultured in vitro,and M1 polarization was induced by 100 ng/mL LPS + 30 ng/mL IFN-γ.The cells were divided into a control group,LPS+IFN group,GDF11 group,and LPS+IFN+GDF11 group.The polarization of the macrophages was detected by flow cytometry,the mRNA expression levels of IL-6,TNF-α,NF-κB,and GDF11 were detected by RT-qPCR,the protein expressions of GDF11,NF-κB,and P-NF-κB were detected by Western blotting,and the protein expression and distribution of GDF11 and NF-κB were detected by immunofluorescence.Results Compared with the control group,the M1 polariza-tion ratio of macrophages in the LPS+IFN group and the expression of IL-6,TNF-α,and NF-κBwere increased,while the expression of GDF11mRNA and protein were decreased;the expression of NF-κB and P-NF-κB proteins and the ratio of P-NF-κB/NF-κB were higher,and NF-κB nucleation was obvious(P<0.05).Compared with the control group,there were no significant changes in the M1 polarization ratio,IL-6,TNF-α,and NF-κBexpression,and P-NF-κB/NF-κB ratio in the GDF11 group(P>0.05).Compared with the LPS+IFN group,the M1 polarization ratio of RAW264.7 macrophages in the LPS+IFN+GDF11 group was significantly reduced,the expression of IL-6,TNF-α,NF-κB mRNA,and NF-κB,P-NF-κB protein was decreased,and the P-NF-κB/NF-κB ratio was also decreased(P<0.05).Conclusion GDF11 alleviates M1 polarization mediated inflammatory response in macrophages,and its mechanism may be related to regulation of the NF-κB pathway.关键词
巨噬细胞/极化/脓毒症/生长转化因子11Key words
macrophage/polarization/sepsis/growth differentiation factor 11分类
医药卫生引用本文复制引用
肖雯,田源,蒋宇,陈芳..GDF11调控NFκ-B通路影响巨噬细胞M1极化介导的炎症反应[J].中国医科大学学报,2024,53(4):342-348,354,8.基金项目
湖南省自然科学基金科药联合项目(2022JJ80065) （2022JJ80065）
湖南省卫生健康委员会一般指导项目(202110000505) （202110000505）
湖南省人民医院仁术国自培育项目(BSJJ202103) （BSJJ202103）
