中国药理学通报2024，Vol.40Issue(4)：645-651,7.DOI:10.12360/CPB202312050
穿山龙薯蓣皂苷通过调控自噬治疗胶原诱导性关节炎小鼠模型的机制
Mechanism research of pangolin diosgenin in treating collagen-induced arthritis mouse model by regulating autophagy
摘要
Abstract
Aim To explore the mechanism of action of pangolin diosgenin in treating collagen-induced arthritis(CIA)model mice through autophagy.Methods CIA mouse model was established using chicken type Ⅱcollagen emulsion.DBA1 mice were initially immu-nized(0.1 mL)and stimulated again with the same dose after 21 days.After boosting the immunization,50 mice immunized with collagen were randomly divid-ed into the CIA model group,tripterygium wilfordii group and pangolin diosgenin low,medium and high dose groups,and another 10 mice were injected with the same amount of normal saline as the control group.After 14 consecutive days of intragastric treatment,the spleen,inguinal lymph nodes and knee joints of CIA mice were removed.The degree of inflammation in the paws of mice in each group was observed.HE staining was performed on ankle joints and knee joints,and re-lated autophagy indicators at mRNA and protein levels in lymph nodes and spleen tissue of each group were detected.Results Compared with the CIA model group,the arthritis index(AI)score of the high-dose pangolin diosgenin group was reduced(P＜0.05);the difference in paw thickness between the tripterygium wilfordii group and the pangolin diosgenin group was reduced(P＜0.01).Compared with the control group,the weight of the CIA model group decreased significantly(P＜0.01).HE staining results of mouse knee joints synovium and paw ankle joints showed that the CIA model group had symptoms of immune cell in-filtration,bone erosion,cartilage destruction,and syn-ovial hyperplasia compared with the control group.The tripterygium wilfordii group and the medium-and high-dose groups of pangolin diosgenin had different degrees of therapeutic effect on the CIA model,and could re-duce and prevent symptoms.Compared with the CIA model group,the mRNA and protein levels of LC3 and Beclin-1 increased in the tripterygium wilfordii group and the pangolin diosgenin-treated group,while the mRNA and protein levels of p62 decreased.Conclu-sion Pangolin diosgenin may have a therapeutic effect on CIA model mice by inhibiting autophagy.关键词
穿山龙薯蓣皂苷/自噬/类风湿性关节炎/胶原诱导性关节炎/p62/LC3/Beclin-1Key words
pangolin diosgenin/autophagy/rheuma-toid arthritis/collagen-induced arthritis/p62/LC3/Beclin-1分类
医药卫生引用本文复制引用
江涛,由涌,夏东帅,薄思涵,王永为,高亚贤,宋鸿儒..穿山龙薯蓣皂苷通过调控自噬治疗胶原诱导性关节炎小鼠模型的机制[J].中国药理学通报,2024,40(4):645-651,7.基金项目
河北省自然科学基金中医药联合基金培育项目(No H2023406057) （No H2023406057）
河北省自然科学基金精准医学联合基金细化项目(No H2021406066) （No H2021406066）
河北省高等学校科学技术研究项目(No ZD2021003) （No ZD2021003）
