中国药理学通报2024，Vol.40Issue(4)：670-678,9.DOI:10.12360/CPB202308057
裙带菜多糖抑制肝癌细胞增殖和迁移的机制
Mechanism of sulfated polysaccharide from Undaria pinnatifida on proliferation and migration of hepatocellular carcinoma cells
摘要
Abstract
Aim To investigate the critical effects of sulfated polysaccharide from Undaria pinnatifida(SP-UP)on hepatocellular carcinoma(HCC)cells and its mechanisms.Methods MTT assay and colony forma-tion assay were used to verify the effect of SPUP on the proliferation of HepG2 and SMMC-7721 cells.The ap-optotic effect of SPUP on HepG2 and SMMC-7721 cells was detected by Hoechst 33258 and Western blot.The metastasis and invasion of HCC cells were detected by Transwell and scratch methods.The autophagy,epi-thelial-mesenchymal transition(EMT),matrix metal-loproteinase 2(MMP-2)and MMP-9 levels in HCC cells were investigated by immunofluorescence and Western blot.Results SPUP had short-term and long-term inhibitory effects on HepG2 and SMMC-7721 cells,minimal inhibitory effect on L-02 cells,and SP-UP could promote apoptosis and autophagy of HCC cells.Meanwhile,SPUP could suppress migration and invasion abilities,EMT and MMPs levels of HCC cells.Conclusions SPUP inhibits the growth and mi-gration of HCC cells via promoting apoptosis,autoph-agy,and inhibiting EMT and MMPs.关键词
裙带菜多糖/肝细胞癌/增殖/自噬/凋亡/侵袭迁移/上皮间质转化/基质金属蛋白酶Key words
sulfated polysaccharide from Undaria pin-natifida/hepatocellular carcinoma/proliferation/auto-phagy/apoptosis/invasion and metastasis/epithelial-mesenchymal transition/matrix metalloproteinase分类
医药卫生引用本文复制引用
刘伟萍,陈建芬,安金琪,罗艺,刘凤斌..裙带菜多糖抑制肝癌细胞增殖和迁移的机制[J].中国药理学通报,2024,40(4):670-678,9.基金项目
国家自然科学基金资助项目(No 81973819,82304982) （No 81973819,82304982）
国家重点研发计划"中医药现代化"重点专项(No 2022YFC3501302) （No 2022YFC3501302）
