中国药理学通报2024，Vol.40Issue(4)：701-709,9.DOI:10.12360/CPB202309032
二十碳五烯酸和二十二碳六烯酸及其组合对斑马鱼阿尔茨海默病模型认知障碍的改善作用和机制
Effect of EPA,DHA and their combination on cognitive impairment in zebrafish AD model and its mechanism
摘要
Abstract
Aim To use the AD model to compare and study the mechanisms of EPA,DHA,the omega-3 pol-yunsaturated fatty acids,and their combination in im-proving cognitive impairment and neuroinflammation.Methods Zebra fish AD model was induced by Al3+,fed with EPA,DHA or EPA+DHA(1:2).New ob-ject recognition(NOR)and T-maze reward were used to evaluated the memory,GC-MS was used to detect PUFAs concentration changes in body,qPCR was ap-plied to detect the changes of AChE,Axl,APP,Bace1,IL-6,TNF-α,CD11b,CD206,VDAC1,caspase-3,caspase-9 and Bax in brain,ELISA kit was employed to detect brain Aβ concentration.Results Compared with the model group,EPA only significant-ly improved zebra fish NOR ability,while DHA and EPA+DHA both restored NOR and memory in the T-maze.EPA,DHA and EPA+DHA significantly inhib-ited Aβ protein concentration change and mRNA ex-pression of IL-6,CD206,caspase-3,caspase-9,Bax,and AChE.EPA and DHA significantly inhibited Bace1,APP,and TNF-α mRNA expression.DHA and EPA+DHA significantly reduced Axl and VDAC1 mRNA expression.Conclusions The combination of EPA and DHA has a better effect on memory and cog-nitive impairment in zebra fish AD model induced by Al3+,and its mechanism may be improving memory and cognitive impairment via inhibiting brain cell apop-tosis.关键词
EPA/DHA/阿尔茨海默病/斑马鱼/认知/记忆/神经炎症Key words
EPA/DHA/Alzheimer's disease/zebra fish/cognition/memory/neuroinflammation分类
医药卫生引用本文复制引用
黄城益,邓淑怡,宋采..二十碳五烯酸和二十二碳六烯酸及其组合对斑马鱼阿尔茨海默病模型认知障碍的改善作用和机制[J].中国药理学通报,2024,40(4):701-709,9.基金项目
广东省基础与应用基础研究基金项目(No 2021A1515011579) （No 2021A1515011579）
深圳市科技计划(国际合作研究)项目(No GJHZ20190823111414825) （国际合作研究）
